Curated repository for small angle scattering data and models


SASBDB currently contains:


3112

experimental data sets

4248

models
545 experimental data sets on hold
701 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDQN4 – Complex of CRISPR associated Lon protease (CalpL) with a 10 kDa C-terminal fragment of CalpT a putative MazF-like toxin (CalpT10)

SAVED domain-containing proteinUncharacterized protein (putative MazF-like toxin) experimental SAS data
CUSTOM IN-HOUSE model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Uncharacterized protein (putative MazF-like toxin) monomer, 9 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 14
Antiviral signaling by a cyclic nucleotide activated CRISPR protease Nature (2022)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter M, Boenigk W, Seifert R, Bode B, Schmid-Burgk J, Svergun D, Geyer M, White M, Hagelueken G
RgGuinier 3.4 nm
Dmax 11.7 nm
VolumePorod 93 nm3

Receptor-type tyrosine-protein phosphatase mu extracellular domains (PTPRM-ECDs)
Receptor-type tyrosine-protein phosphatase mu experimental SAS data
Determinants of receptor tyrosine phosphatase homophilic adhesion: structural comparison of PTPRK and PTPRM extracellular domains Journal of Biological Chemistry 2022 Nov :102750
Journal of Biological Chemistry :102750 (2022 Nov)
Doubly Phosphorylated p190RhoGAP Peptide Bound to p120RasGAP's SH2-SH3-SH2 Domains
Ras GTPase-activating protein 1Rho GTPase-activating protein 35 experimental SAS data
Tandem engagement of phosphotyrosines by the dual SH2 domains of p120RasGAP. Structure 2022 Nov 16;
Structure (2022 Nov 16)
The N-terminal domain of Phospholipase A and acyltransferase 4 (PLAAT4 NTD)
Phospholipase A and acyltransferase 4 experimental SAS data
Crystal structure of the phospholipase A and acyltransferase 4 (PLAAT4) catalytic domain. J Struct Biol 2022 Oct 7;214(4):107903
J Struct Biol 214(4):107903 (2022 Oct 7)
Phosphoprotein of Borna disease virus
Phosphoprotein experimental SAS data
Borna Disease Virus 1 Phosphoprotein Forms a Tetramer and Interacts with Host Factors Involved in DNA Double-Strand Break Repair and mRNA Processing. Viruses 2022 Oct 26;14(11)
Viruses 14(11) (2022 Oct 26)
Sliding beta clamp
Beta sliding clamp experimental SAS data
Regulation of futile ligation during early steps of BER in M. tuberculosis is carried out by a β-clamp-XthA-LigA tri-component complex International Journal of Biological Macromolecules 2022 Nov
International Journal of Biological Macromolecules (2022 Nov)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.