Browse by MACROMOLECULE type: protein

SASDHN4 – N-terminal 5 domains of the cation-independent mannose-6-phosphate receptor (CI-MPR) bound to mannose 6-phosphate (M6P)

Cation-independent mannose-6-phosphate receptor experimental SAS data
Cation-independent mannose-6-phosphate receptor Kratky plot
Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein
Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12
Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor. Commun Biol 3(1):498 (2020)
Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM
RgGuinier 3.7 nm
Dmax 13.3 nm
VolumePorod 150 nm3

SASDHP4 – Palmitoyl-protein thioesterase 1 (PPT1)

Palmitoyl-protein thioesterase 1 experimental SAS data
Palmitoyl-protein thioesterase 1 Kratky plot
Sample: Palmitoyl-protein thioesterase 1 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12
Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor. Commun Biol 3(1):498 (2020)
Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM
RgGuinier 2.3 nm
Dmax 9.8 nm
VolumePorod 54 nm3

SASDHQ4 – N-terminal domains 1-5 of the cation-independent mannose-6-phosphate receptor (CI-MPR) in complex with palmitoyl-protein thioesterase 1 (PPT1)

Cation-independent mannose-6-phosphate receptorPalmitoyl-protein thioesterase 1 experimental SAS data
Cation-independent mannose-6-phosphate receptor Palmitoyl-protein thioesterase 1 Kratky plot
Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein
Palmitoyl-protein thioesterase 1 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12
Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor. Commun Biol 3(1):498 (2020)
Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM
RgGuinier 4.9 nm
Dmax 19.3 nm
VolumePorod 258 nm3

SASDG54 – Human macrophage mannose receptor 1 protein

Macrophage mannose receptor 1 experimental SAS data
ITASSER model
Sample: Macrophage mannose receptor 1 dimer, 315 kDa Mouse myeloma cell … protein
Buffer: 50mM Hepes, 100mM NaCl, 1mM DTT, pH: 7
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 15
Mannose receptor (CD206) activation in tumor-associated macrophages enhances adaptive and innate antitumor immune responses. Sci Transl Med 12(530) (2020)
Jaynes JM, Sable R, Ronzetti M, Bautista W, Knotts Z, Abisoye-Ogunniyan A, Li D, Calvo R, Dashnyam M, Singh A, Guerin T, White J, Ravichandran S, Kumar P, Talsania K, Chen V, Ghebremedhin A, Karanam B, Bin Salam A, Amin R, Odzorig T, Aiken T, Nguyen V, Bian Y, Zarif JC, de Groot AE, Mehta M, Fan L, Hu X, Simeonov A, Pate N, Abu-Asab M, Ferrer M, Southall N, Ock CY, Zhao Y, Lopez H, Kozlov S, de Val N, Yates CC, Baljinnyam B, Marugan J, Rudloff U
RgGuinier 7.9 nm
Dmax 30.1 nm
VolumePorod 584 nm3

SASDMG8 – Splicing factor, proline- and glutamine-rich-QM bound to Non-POU domain-containing octamer-binding protein: SFPQ(276-598)-QM / NONO(53-312) heterodimer

Non-POU domain-containing octamer-binding proteinSplicing factor, proline- and glutamine-rich experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Non-POU domain-containing octamer-binding protein monomer, 30 kDa Homo sapiens protein
Splicing factor, proline- and glutamine-rich monomer, 38 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 5
Structural basis of the zinc-induced cytoplasmic aggregation of the RNA-binding protein SFPQ Nucleic Acids Research 48(6):3356-3365 (2020)
Huang J, Ringuet M, Whitten A, Caria S, Lim Y, Badhan R, Anggono V, Lee M
RgGuinier 4.0 nm
Dmax 16.5 nm
VolumePorod 90 nm3

SASDMH8 – Splicing factor, proline- and glutamine-rich-QM-TM bound to Non-POU domain-containing octamer-binding protein: SFPQ(276-598)-QM-TM / NONO(52-312) heterodimer

Splicing factor, proline- and glutamine-richNon-POU domain-containing octamer-binding protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Splicing factor, proline- and glutamine-rich monomer, 38 kDa Homo sapiens protein
Non-POU domain-containing octamer-binding protein monomer, 30 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 8
Structural basis of the zinc-induced cytoplasmic aggregation of the RNA-binding protein SFPQ Nucleic Acids Research 48(6):3356-3365 (2020)
Huang J, Ringuet M, Whitten A, Caria S, Lim Y, Badhan R, Anggono V, Lee M
RgGuinier 3.6 nm
Dmax 16.5 nm
VolumePorod 80 nm3

SASDHF4 – Lipoprotein lipase-GPIHBP1-monoclonal antibody (5D2) complex

Lipoprotein lipaseGlycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1Monoclonal Antibody Fragment 5D2 experimental SAS data
CORAL model
Sample: Lipoprotein lipase monomer, 50 kDa Homo sapiens protein
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 monomer, 15 kDa Homo sapiens protein
Monoclonal Antibody Fragment 5D2 monomer, 47 kDa Mus musculus protein
Buffer: 10 mM Tris, 150 mM NaCl, 4 mM CaCL2, 10% (v/v) Glycerol, 0.05% 0.8mM CHAPS, ,0.05 % (v/v) NaN3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 14
Unfolding of monomeric lipoprotein lipase by ANGPTL4: Insight into the regulation of plasma triglyceride metabolism Proceedings of the National Academy of Sciences :201920202 (2020)
Kristensen K, Leth-Espensen K, Mertens H, Birrane G, Meiyappan M, Olivecrona G, Jørgensen T, Young S, Ploug M
RgGuinier 4.9 nm
Dmax 17.5 nm
VolumePorod 187 nm3

SASDG55 – Deglycosylated latency associated peptide, LAP (TGFB-1)

Latency associated peptide experimental SAS data
Latency associated peptide Kratky plot
Sample: Latency associated peptide dimer, 58 kDa Homo sapiens protein
Buffer: phosphate buffered saline 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 20
Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states IUCrJ 7(2) (2020)
Stachowski T, Snell M, Snell E
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 129 nm3

SASDMJ4 – Lysozyme amyloid fibril (LAF)

lysozyme amyloid fibril experimental SAS data
DAMMIF model
Sample: Lysozyme amyloid fibril, 14 kDa Gallus gallus protein
Buffer: 0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 5
Effect of the concentration of protein and nanoparticles on the structure of biohybrid nanocomposites. Biopolymers 111(2):e23342 (2020)
Majorošová J, Schroer MA, Tomašovičová N, Batková M, Hu PS, Kubovčíková M, Svergun DI, Kopčanský P
RgGuinier 30.2 nm
Dmax 120.0 nm

SASDML4 – LAF + MNP (r = 5.6 nm) nanocomposite

lysozyme amyloid fibrilFe3O4 nanoparticles; radius 5.6 nm (AFM based) experimental SAS data
DAMMIF model
Sample: Lysozyme amyloid fibril, 1 kDa Gallus gallus protein
Fe3O4 nanoparticles; radius 5.6 nm (AFM based) monomer, 1 kDa
Buffer: 0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 8
Effect of the concentration of protein and nanoparticles on the structure of biohybrid nanocomposites. Biopolymers 111(2):e23342 (2020)
Majorošová J, Schroer MA, Tomašovičová N, Batková M, Hu PS, Kubovčíková M, Svergun DI, Kopčanský P
RgGuinier 29.4 nm
Dmax 80.0 nm