Themis

Yanbo Zhao.

SASD2S8 – Themis wild type

Human thymocyte-expressed molecule involved in selection
MWexperimental 55 kDa
MWexpected 64 kDa
log I(s) 1.21×101 1.21×100 1.21×10-1 1.21×10-2
Human thymocyte-expressed molecule involved in selection small angle scattering data  s, nm-1
ln I(s)
Human thymocyte-expressed molecule involved in selection Guinier plot ln 1.21×101 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Human thymocyte-expressed molecule involved in selection Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Themis wild type in 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF) storage ring (Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 30 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Human thymocyte-expressed molecule involved in selection (Themis)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   64.3 kDa
 
UniProt   Q8N1K5 (1-561)
Sequence   FASTA