Themis

Yanbo Zhao.

SASD2V8 – Themis Y541F point mutation

Thymocyte-expressed molecule involved in selection Y541F
MWexperimental 56 kDa
MWexpected 64 kDa
log I(s) 1.68×101 1.68×100 1.68×10-1 1.68×10-2
Thymocyte-expressed molecule involved in selection Y541F small angle scattering data  s, nm-1
ln I(s)
Thymocyte-expressed molecule involved in selection Y541F Guinier plot ln 1.68×101 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Thymocyte-expressed molecule involved in selection Y541F Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Themis Y541F point mutation in 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF) storage ring (Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 30 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Thymocyte-expressed molecule involved in selection Y541F
Mol. type   Protein
Olig. state   Monomer
Mon. MW   64.4 kDa
 
UniProt   Q8N1K5 (1-561)
Sequence   FASTA