Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations.

Kulakova A, Indrakumar S, S√łnderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
Sci Rep 10(1):10089 (2020 Jun 22)
PMID: 32572086
doi: 10.1038/s41598-020-67002-9
Submitted to SASBDB: 2020 Mar 20
Published in SASBDB:

SASDHV8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.0

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 267 nm3

SASDHW8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 274 nm3

SASDHX8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 6.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 258 nm3

SASDHY8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 7.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 26
RgGuinier 5.0 nm
Dmax 17.4 nm
VolumePorod 270 nm3

SASDHZ8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM TRIS, pH 8.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM TRIS, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 15
RgGuinier 4.9 nm
Dmax 16.7 nm
VolumePorod 239 nm3

SASDH29 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM phosphate, pH 6.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM phosphate, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7
RgGuinier 4.9 nm
Dmax 16.7 nm
VolumePorod 240 nm3