Autoinhibition of untimely DNA binding by structural disorder in the full-length peroxisome proliferator-activated receptors (PPARs)

Lalic M, Thomsen E, Thomasen F, Udupa A, Salehian Andersen E, Kuppermann S, Christensen C, Holm-Olesen E, Saar D, Prestel A, Bauer L, McKenna J, Olsen J, Mandrup S, Hartmann-Petersen R, Jeffries C, Staller M, Lindorff-Larsen K, Kragelund B
Nature Communications (2026 Aug 26)

doi: 10.1038/s41467-026-76962-x

Submitted to SASBDB: 2025 Jan 6 Published in SASBDB: 2026 Aug 18

SASDWX3 – Full length peroxisome proliferator-activated receptor gamma (PPARγ) Sample: Peroxisome proliferator-activated receptor gamma monomer, 58 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM KCl, 1 mM TCEP, 2% v/v glycerol, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 12 RgGuinier 3.7 nm Dmax 16.5 nm VolumePorod 123 nm3

SASDWY3 – N-terminal truncation of peroxisome proliferator-activated receptor gamma (ΔAB-PPARγ) Sample: Peroxisome proliferator-activated receptor gamma monomer, 43 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 5 mM NaNO3, 5 mM TCEP, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 20 RgGuinier 2.4 nm Dmax 8.4 nm VolumePorod 71 nm3

SASDWZ3 – The intrinsically disordered N-terminal AB domain of peroxisome proliferator-activated receptor gamma (PPARγ-AB) Sample: Peroxisome proliferator-activated receptor gamma monomer, 15 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 5 mM NaNO3, 5 mM TCEP, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 16 RgGuinier 3.5 nm Dmax 16.0 nm VolumePorod 21 nm3

SASDW24 – The N-terminal AB domain and DNA binding domain of peroxisome proliferator-activated receptor alpha (PPARα-ABDBD) Sample: Peroxisome proliferator-activated receptor alpha monomer, 20 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 5 mM NaNO3, 5 mM TCEP, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 16 RgGuinier 2.4 nm Dmax 10.3 nm VolumePorod 37 nm3

SASDW34 – The intrinsically disordered N-terminal AB domain of peroxisome proliferator-activated receptor alpha (PPARα-AB) Sample: Peroxisome proliferator-activated receptor alpha monomer, 10 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 5 mM NaNO3, 5 mM TCEP, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 16 RgGuinier 3.0 nm Dmax 11.0 nm VolumePorod 16 nm3