AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline.
Da Vela S,
Bartels K,
Franke D,
Soloviov D,
Gräwert T,
Molodenskiy D,
Kolb B,
Wilhelmy C,
Drexel R,
Meier F,
Haas H,
Langguth P,
Graewert MA
J Synchrotron Radiat
(2025 Jul 1)
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Sample: |
Ferritin light chain 24-mer, 480 kDa Equus caballus protein
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Buffer: |
phosphate buffered saline, pH: 7.2 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Jun 19
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RgGuinier |
5.4 |
nm |
Dmax |
13.3 |
nm |
VolumePorod |
632 |
nm3 |
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Sample: |
Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae (strain … protein
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Buffer: |
phosphate buffered saline, 1% glycerol, pH: 7.2 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Apr 13
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RgGuinier |
3.4 |
nm |
Dmax |
9.5 |
nm |
VolumePorod |
197 |
nm3 |
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Sample: |
Beta-amylase tetramer, 224 kDa Ipomoea batatas protein
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Buffer: |
phosphate buffered saline, 1% glycerol, pH: 7.2 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Apr 13
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RgGuinier |
4.2 |
nm |
Dmax |
12.6 |
nm |
VolumePorod |
297 |
nm3 |
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Sample: |
Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm monomer, 0 kDa synthetic construct
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Buffer: |
water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Jun 19
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RgGuinier |
47.7 |
nm |
Dmax |
111.0 |
nm |
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Sample: |
Thermo Fisher polystyrene nanospheres with a diameter of 20 nm monomer, 1 kDa synthetic construct
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Buffer: |
water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Jun 19
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RgGuinier |
9.0 |
nm |
Dmax |
25.0 |
nm |
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