AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline.

Da Vela S, Bartels K, Franke D, Soloviov D, Gräwert T, Molodenskiy D, Kolb B, Wilhelmy C, Drexel R, Meier F, Haas H, Langguth P, Graewert MA
J Synchrotron Radiat (2025 Jul 1)

PMID: 40503919 doi: 10.1107/S1600577525003959

Submitted to SASBDB: 2024 Dec 31 Published in SASBDB: 2025 Jun 7

SASDW94 – Horse apoferritin from AF4-SAXS measurements Sample: Ferritin light chain 24-mer, 480 kDa Equus caballus protein Buffer: phosphate buffered saline, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19 RgGuinier 5.4 nm Dmax 13.3 nm VolumePorod 632 nm3

SASDWA4 – Yeast alcohol dehydrogenase 1 from AF4-SAXS measurements Sample: Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae (strain … protein Buffer: phosphate buffered saline, 1% glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13 RgGuinier 3.4 nm Dmax 9.5 nm VolumePorod 197 nm3

SASDWB4 – Sweet potato beta-amylase from AF4-SAXS measurement Sample: Beta-amylase tetramer, 224 kDa Ipomoea batatas protein Buffer: phosphate buffered saline, 1% glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13 RgGuinier 4.2 nm Dmax 12.6 nm VolumePorod 297 nm3

SASDWC4 – 100 nm polystyrene beads from AF4-SAXS measurements Sample: Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm monomer, 0 kDa synthetic construct Buffer: water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19 RgGuinier 47.7 nm Dmax 111.0 nm

SASDWD4 – 20 nm polystyrene beads from AF4-SAXS measurements Sample: Thermo Fisher polystyrene nanospheres with a diameter of 20 nm monomer, 1 kDa synthetic construct Buffer: water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19 RgGuinier 9.0 nm Dmax 25.0 nm