Characterizing two nanobodies for SMAD4.

Condeminas M, Torner C, Pluta R, Ruiz L, R de Régil M, Khan RJ, Pous J, Pardon E, Steyaert J, Fromm S, Martin-Malpartida P, Macias MJ
FEBS Lett (2026 Sep 24)

PMID: 42779504 doi: 10.1002/1873-3468.70466

Submitted to SASBDB: 2025 Mar 5 Published in SASBDB: 2026 Sep 28

SASDWQ9 – Mothers against decapentaplegic homolog 4 (SMAD4) C-terminal domain (amino acids 272-552, S4SADMH2) Sample: Mothers against decapentaplegic homolog 4 monomer, 31 kDa Homo sapiens protein Buffer: 20 mM TRIS, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2024 Apr 20 RgGuinier 2.5 nm Dmax 9.0 nm VolumePorod 45 nm3

SASDWV9 – Mothers against decapentaplegic homolog 3 (SMAD3) C-terminal domain with phosphomimetic mutations Ser423Asp and Ser425Asp (amino acids 189-552, S3MH2DVD) Sample: Mothers against decapentaplegic homolog 3 (S423D, S425D) trimer, 80 kDa Homo sapiens protein Buffer: 20 mM TRIS, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2024 Apr 20 RgGuinier 4.0 nm Dmax 22.0 nm VolumePorod 202 nm3

SASDWW9 – SMAD4 C-terminal domain (amino acids 272-552, S4SADMH2) bound to SMAD3 C-terminal domain with phosphomimetic mutations Ser423Asp and Ser425Asp (amino acids 189-425, S3MH2DVD) Sample: Mothers against decapentaplegic homolog 4 monomer, 31 kDa Homo sapiens protein Mothers against decapentaplegic homolog 3 (S423D, S425D) dimer, 53 kDa Homo sapiens protein Buffer: 20 mM TRIS, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2024 Apr 20 RgGuinier 3.6 nm Dmax 19.0 nm VolumePorod 131 nm3

SASDWT9 – Megabody derived from NbA7 and YgjK (MbA7-YgjK) Sample: Megabody derived from NbA7 and YgjK monomer, 100 kDa Lama glama and … protein Buffer: 20 mM TRIS, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2024 Apr 20 RgGuinier 3.2 nm Dmax 10.0 nm VolumePorod 126 nm3

SASDWU9 – Megabody derived from NbA7 and YgjK (MbA7-YgjK) bound to Mothers against decapentaplegic homolog 4 (SMAD4) C-terminal domain (amino acids 272-552, S4SADMH2) Sample: Mothers against decapentaplegic homolog 4 monomer, 31 kDa Homo sapiens protein Megabody derived from NbA7 and YgjK monomer, 100 kDa Lama glama and … protein Buffer: 20 mM TRIS, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2024 Apr 20 RgGuinier 4.1 nm Dmax 22.0 nm VolumePorod 213 nm3

SASDWX9 – Megabody derived from NbA7 and YgjK (MbA7-YgjK) complexed to SMAD4 C-terminal domain (amino acids 272-552, S4SADMH2) bound to SMAD3 C-terminal domain with phosphomimetic mutations Ser423Asp and Ser425Asp (amino acids 189-425, S3MH2DVD) Sample: Mothers against decapentaplegic homolog 4 monomer, 31 kDa Homo sapiens protein Megabody derived from NbA7 and YgjK monomer, 100 kDa Lama glama and … protein Mothers against decapentaplegic homolog 3 (S423D, S425D) dimer, 53 kDa Homo sapiens protein Buffer: 20 mM TRIS, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2024 Apr 20 RgGuinier 5.0 nm Dmax 25.0 nm VolumePorod 261 nm3

SASDWR9 – Megabody derived from NbA1 and cHopQ (MbA1-cHopQ) Sample: Megabody derived from NbA1 and cHopQ monomer, 56 kDa Lama glama and … protein Buffer: 20 mM TRIS, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2024 Apr 20 RgGuinier 3.6 nm Dmax 18.0 nm VolumePorod 84 nm3

SASDWS9 – Megabody derived from NbA1 and cHopQ (MbA1-cHopQ) bound to Mothers against decapentaplegic homolog 4 (SMAD4) C-terminal domain (amino acids 272-552, S4SADMH2) Sample: Mothers against decapentaplegic homolog 4 monomer, 31 kDa Homo sapiens protein Megabody derived from NbA1 and cHopQ monomer, 56 kDa Lama glama and … protein Buffer: 20 mM TRIS, 200 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2024 Apr 20 RgGuinier 4.4 nm Dmax 22.0 nm VolumePorod 170 nm3