EFAMIX , a tool to decompose inline chromatography SAXS data from partially overlapping components

Konarev P, Graewert M, Jeffries C, Fukuda M, Cheremnykh T, Volkov V, Svergun D
Protein Science (2021 Nov 22)

doi: 10.1002/pro.4237
Submitted to SASBDB: 2021 Jan 17
Published in SASBDB:

SASDEX9 – Class II pyruvate aldolase (HpcH/HpaI aldolase), wild-type hexamer in HEPES isolated using SEC-SAXS

HpcH/HpaI aldolase experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase hexamer, 165 kDa Sphingomonas wittichii protein
Buffer: 20 mM HEPES,, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
RgGuinier 3.3 nm
Dmax 9.4 nm
VolumePorod 233 nm3