{"code":"SASDA89","status":"Published","type_of_curve":"Other","angular_unit":"1/nm","project":{"title":"Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF.","publication":{"title":"Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF.","author_list":"Felix J, Kandiah E, De Munck S, Bloch Y, van Zundert GC, Pauwels K, Dansercoer A, Novanska K, Read RJ, Bonvin AM, Vergauwen B, Verstraete K, Gutsche I, Savvides SN","journal":"Nat Commun","doi":"10.1038/ncomms13228","pmid":"27819269","published_date":"2016 Nov 7"},"status":"released","submitted_date":"2015-08-07","released_date":"2016-12-16"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDA89.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDA89.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA89_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA89_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDA89_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA89_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDA89.sascif","experiment":{"instrument":{"detector":{"type":"CCD","name":"AVIEX PCCD170170","resolution":null},"name":"SOLEIL","city":"Saint-Aubin","country":"France","beamline_name":"SWING","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"GM-CSF/IL-2 inhibition factor","short_name":null,"sequence":"MACLRVFLAVLALCGSVHSAQWIGERDFCTAHAQDVFARLQVWMRIDRNVTAADNSSACALAIETPPSNFDADVYVAAAGINVSVSAINCGFFNMRQVETTYNTARRQMYVYMDSWDPWVIDDPQPLFSQEYENETLPYLLEVLELARLYIRVGCTVPGEQPFEVIPGIDYPHTGMEFLQHVLRPNRRFAPAKLHMDLEVDHRCVSAVHVKAFLQDACSARKARTPLYFAGHGCNHPDRRPKNPVPRPQHVSSPISRKCSMQTAR","organism":"Orf virus","uniprot_code":"Q9J5U5","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MACLRVFLAVLALCGSVHSAQWIGERDFCTAHAQDVFARLQVWMRIDRNVTAADNSSACA\nLAIETPPSNFDADVYVAAAGINVSVSAINCGFFNMRQVETTYNTARRQMYVYMDSWDPWV\nIDDPQPLFSQEYENETLPYLLEVLELARLYIRVGCTVPGEQPFEVIPGIDYPHTGMEFLQ\nHVLRPNRRFAPAKLHMDLEVDHRCVSAVHVKAFLQDACSARKARTPLYFAGHGCNHPDRR\nPKNPVPRPQHVSSPISRKCSMQTAR","mw":29.924,"total_mw":119.696,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Granulocyte-macrophage colony-stimulating factor","short_name":null,"sequence":"APTRQPSPVTRPWQHVDAIKEALSLLNDSTDTAAVMDETVEVVSEMFDSQEPTCLQTRLE\r\nLYKQGLRGSLTSLTGSLTMMASHYKKHCPPTQETSCETQIITFKSFKENLKDFLFIIPFD\r\nCWEPVQK","organism":"Ovis aries","uniprot_code":"P28773","uniprot_range_first":18,"uniprot_range_last":144,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MWLQNLLLLGTVVCSFSAPTRQPSPVTRPWQHVDAIKEALSLLNDSTDTAAVMDETVEVV\nSEMFDSQEPTCLQTRLELYKQGLRGSLTSLTGSLTMMASHYKKHCPPTQETSCETQIITF\nKSFKENLKDFLFIIPFDCWEPVQK","mw":14.411,"total_mw":28.823,"number_molecules":2,"complex_state":true,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES 150 mM NaCl","concentration_unit":"mM","comment":null,"additive":"150 mM NaCl","concentration":20.0,"pka":null,"ph":7.4,"deuteration":null},"purity_method":"SEC-SAXS","name":"Complex between ovine GM-CSF and GM-CSF/IL-2 inhibition factor","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[],"contributor_name":"Jan","contributor_surname":"Felix","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2014-09-10","storage_temperature":null,"cell_temperature":20.0,"exposure_time":null,"number_of_frames":16,"wavelength":0.1,"sample_detector_distance":1.82,"concentration_min":null,"concentration_max":9.7,"sample_volume":null,"flow_rate":null,"s_min":0.076,"s_max":5.496,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SAXS_MODEL_GIF_GMCSF_img.png","software":"FoXS","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDA89_fit1_model1.pdb","model_mw":102.1,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":29}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA89_fit1_fit_img.png","software":null,"chi_square_value":99.0,"p_value":0.0,"fit_residual_plot":"SASDA89_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDA89_fit1.fit","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":"Modeling of SAXS data:  The crystal structure of the GIF:oGM-CSF complex was used as an input for the Allosmod-FoXS web server to model missing loops, N- and C-termini and N-linked glycosylation. During each Allosmod-FoXS run, data up to a scattering angle of 0.5 Å-1 was used. Fits to the experimental SAXS data were calculated using the FoXS software.","experiment_description":"X-ray synchrotron radiation scattering data from solutions of the complex between GM-CSF/IL-2 inhibition factor and ovine Granulocyte-macrophage colony-stimulating factor in 20 mM HEPES 150 mM NaCl were collected on the SWING camera on the storage ring SOLEIL (Saint-Aubin, France) using a CCD AVIEX detector (I(s) vs s, where s = 4π sin θ/λ, where 2θ is the scattering angle). One solute concentration of 10 mg/ml was measured. 10 successive frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The purity of the sample was obtained via SEC-SAXS.","tags":[],"intensity_unit":null,"experimental_mw":153.0,"experimental_mw_error":null,"guinier_i0_mw":153.0,"guinier_i0_mw_error":null,"porod_mw":144.0,"porod_mw_error":null,"pddf_i0":0.1494,"pddf_i0_error":null,"guinier_i0":0.151381,"guinier_i0_error":0.0001,"pddf_rg":3.77,"pddf_rg_error":null,"guinier_rg":3.79,"guinier_rg_error":0.13,"pddf_dmax":12.4,"pddf_dmax_error":null,"porod_volume":231.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":17,"guinier_point_last":47,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDA89_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}