{"code":"SASDAK8","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.","publication":{"title":"Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.","author_list":"Banerjee A, Herman E, Kottke T, Essen LO","journal":"Structure","doi":"10.1016/j.str.2015.10.022","pmid":"26688213","published_date":"2016 Jan 5"},"status":"released","submitted_date":"2015-06-18","released_date":"2016-01-05"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDAK8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDAK8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAK8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAK8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDAK8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAK8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDAK8.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Aureochrome1a-A´α-LOV-Jα","short_name":"Aureochrome1a-A","sequence":"MGSSHHHHHHSSGLVPRGSHMDFSFIKALQTAQQNFVVTDPSLPDNPIVYASQGFLNLTGYSLDQILGRNCRFLQGPETDPKAVERIRKAIEQGNDMSVCLLNYRVDGTTFWNQFFIAALRDAGGNVTNFVGVQCKVSDQYAATVTKQQEEEEEAAANDDED\r\n","organism":"Phaeodactylum tricornutum","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":null,"mw":17.973,"total_mw":35.946,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"10 mM Tris 300 mM NaCl","concentration_unit":"mM","comment":null,"additive":"300 mM NaCl","concentration":10.0,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Dark state solution structure of Aureochrome1a- A´α-LOV-Jα ","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Marburg, Germany","full_name":"Philipps-Universität Marburg","webpage":""}],"contributor_name":"Ankan","contributor_surname":"Banerjee","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-03-16","storage_temperature":4.0,"cell_temperature":null,"exposure_time":20.0,"number_of_frames":10,"wavelength":0.93,"sample_detector_distance":2.43,"concentration_min":0.75,"concentration_max":15.0,"sample_volume":null,"flow_rate":null,"s_min":0.034,"s_max":4.975,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDAK8_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDAK8_fit1_model1.pdb","model_mw":47.51,"bead_radius":3.25,"log":null,"symmetry":"","comment":"The model is an averaged and volume corrected representation (DAMFILT)","user":72}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDAK8_fit1_fit_img.png","software":null,"chi_square_value":0.595,"p_value":0.000373,"fit_residual_plot":"SASDAK8_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDAK8_fit1.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":"Solution scattering of N-terminally His6 tagged A´α-LOV-Jα was measured in the dark state (under safe red light). Two concentrations of the sample were measured at 40 and 833 micromolar and then merged. Radiation damage was observed at very low extent. The data were normalized and the buffer only scattering subtracted. The analysis was performed using ATSAS. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.","experiment_description":null,"tags":[],"intensity_unit":null,"experimental_mw":43.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":42.6,"pddf_i0_error":null,"guinier_i0":42.33,"guinier_i0_error":null,"pddf_rg":3.28,"pddf_rg_error":null,"guinier_rg":3.16,"guinier_rg_error":null,"pddf_dmax":13.0,"pddf_dmax_error":null,"porod_volume":67.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":17,"guinier_point_last":71,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDAK8_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}