{"code":"SASDB25","status":"Published","type_of_curve":"Extrapolated to infinite dilution","angular_unit":"1/nm","project":{"title":"Clinically Linked Mutations in the Central Domains of Cardiac Myosin-Binding Protein C with Distinct Phenotypes Show Differential Structural Effects.","publication":{"title":"Clinically Linked Mutations in the Central Domains of Cardiac Myosin-Binding Protein C with Distinct Phenotypes Show Differential Structural Effects.","author_list":"Nadvi NA, Michie KA, Kwan AH, Guss JM, Trewhella J","journal":"Structure","doi":"10.1016/j.str.2015.11.001","pmid":"26688216","published_date":"2016 Jan 5"},"status":"released","submitted_date":"2016-06-25","released_date":"2017-12-12"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDB25.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDB25.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB25_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB25_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB25_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB25_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDB25.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Australian  Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":"Point","type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Cardiac myosin binding protein-C: domains C5-C6-C7","short_name":"cMyBP-C","sequence":"GPGSRQEPPKIHLDCPGRIPDTIVVVAGNKLRLDVPISGDPAPTVIWQKAITQGNKAPARPAPDAPEDTGDSDEWVFDKKLLCETEGRVRVETTKDRSIFTVEGAEKEDEGVYTVTVKNPVGEDQVNLTVKVIDVPDAPAAPKISNVGEDSCTVQWEPPAYDGGQPILGYILERKKKKSYRWMRLNFDLIQELSHEARRMIEGVVYEMRVYAVNAIGMSRPSPASQPFMPIGPPSEPTHLAVEDVSDTTVSLKWRPPERVGAGGLDGYSVEYCPEGCSEWVAALQGLTEHTSILVKDLPTGARLLFRVRAHNMAGPGAPVTTTEPVTV","organism":"Homo sapiens","uniprot_code":"Q14896","uniprot_range_first":641,"uniprot_range_last":964,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MPEPGKKPVSAFSKKPRSVEVAAGSPAVFEAETERAGVKVRWQRGGSDISASNKYGLATE\nGTRHTLTVREVGPADQGSYAVIAGSSKVKFDLKVIEAEKAEPMLAPAPAPAEATGAPGEA\nPAPAAELGESAPSPKGSSSAALNGPTPGAPDDPIGLFVMRPQDGEVTVGGSITFSARVAG\nASLLKPPVVKWFKGKWVDLSSKVGQHLQLHDSYDRASKVYLFELHITDAQPAFTGSYRCE\nVSTKDKFDCSNFNLTVHEAMGTGDLDLLSAFRRTSLAGGGRRISDSHEDTGILDFSSLLK\nKRDSFRTPRDSKLEAPAEEDVWEILRQAPPSEYERIAFQYGVTDLRGMLKRLKGMRRDEK\nKSTAFQKKLEPAYQVSKGHKIRLTVELADHDAEVKWLKNGQEIQMSGSKYIFESIGAKRT\nLTISQCSLADDAAYQCVVGGEKCSTELFVKEPPVLITRPLEDQLVMVGQRVEFECEVSEE\nGAQVKWLKDGVELTREETFKYRFKKDGQRHHLIINEAMLEDAGHYALCTSGGQALAELIV\nQEKKLEVYQSIADLMVGAKDQAVFKCEVSDENVRGVWLKNGKELVPDSRIKVSHIGRVHK\nLTIDDVTPADEADYSFVPEGFACNLSAKLHFMEVKIDFVPRQEPPKIHLDCPGRIPDTIV\nVVAGNKLRLDVPISGDPAPTVIWQKAITQGNKAPARPAPDAPEDTGDSDEWVFDKKLLCE\nTEGRVRVETTKDRSIFTVEGAEKEDEGVYTVTVKNPVGEDQVNLTVKVIDVPDAPAAPKI\nSNVGEDSCTVQWEPPAYDGGQPILGYILERKKKKSYRWMRLNFDLIQELSHEARRMIEGV\nVYEMRVYAVNAIGMSRPSPASQPFMPIGPPSEPTHLAVEDVSDTTVSLKWRPPERVGAGG\nLDGYSVEYCPEGCSEWVAALQGLTEHTSILVKDLPTGARLLFRVRAHNMAGPGAPVTTTE\nPVTVQEILQRPRLQLPRHLRQTIQKKVGEPVNLLIPFQGKPRPQVTWTKEGQPLAGEEVS\nIRNSPTDTILFIRAARRVHSGTYQVTVRIENMEDKATLVLQVVDKPSPPQDLRVTDAWGL\nNVALEWKPPQDVGNTELWGYTVQKADKKTMEWFTVLEHYRRTHCVVPELIIGNGYYFRVF\nSQNMVGFSDRAATTKEPVFIPRPGITYEPPNYKALDFSEAPSFTQPLVNRSVIAGYTAML\nCCAVRGSPKPKISWFKNGLDLGEDARFRMFSKQGVLTLEIRKPCPFDGGIYVCRATNLQG\nEARCECRLEVRVPQ","mw":35.751,"total_mw":35.751,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris-HCl, 250 mM NaCl, 2 mM TCEP, 0.02% sodium azide","concentration_unit":"mM","comment":null,"additive":"250 mM NaCl, 2 mM TCEP, 0.02% sodium azide","concentration":25.0,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Cardiac myosin binding protein-C: domains C5-C6-C7","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Sydney, New South Wales 2006, Australia","full_name":"School of Molecular Bioscience, University of Sydney.","webpage":""}],"contributor_name":"Jill","contributor_surname":"Trewhella","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2015-04-18","storage_temperature":22.0,"cell_temperature":22.0,"exposure_time":1.0,"number_of_frames":44,"wavelength":null,"sample_detector_distance":1.6,"concentration_min":0.7,"concentration_max":3.4,"sample_volume":null,"flow_rate":null,"s_min":0.057,"s_max":3.436,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from solutions of human cardiac myosin binding protein-C, domains C5-C6-C7 in 25 mM TrisHCl 250 mM NaCl, 2 mM TCEP, 0.02% sodium azide were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M-W pixel detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.10332 nm). Different solute concentrations in the range 0.70-3.40 mg/ml were measured using a total exposure time of 44 s (recorded as 44 x 1 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data presented here represent the SAXS profile extrapolated to zero concentration calculated from the solute concentration series.","tags":[],"intensity_unit":null,"experimental_mw":38.2,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.1068,"pddf_i0_error":null,"guinier_i0":0.1055,"guinier_i0_error":0.0001,"pddf_rg":3.931,"pddf_rg_error":0.001,"guinier_rg":3.75,"guinier_rg_error":0.01,"pddf_dmax":14.1,"pddf_dmax_error":null,"porod_volume":54.7,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":15,"guinier_point_last":75,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDB25_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}