{"code":"SASDB27","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA.","publication":{"title":"Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA.","author_list":"Arentson BW, Hayes EL, Zhu W, Singh H, Tanner JJ, Becker DF","journal":"Biosci Rep","doi":"10.1042/BSR20160435","pmid":"27742866","published_date":"2016 Dec"},"status":"released","submitted_date":"2016-10-10","released_date":"2016-10-16"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDB27.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDB27.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB27_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB27_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB27_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB27_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDB27.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"MAR 165 CCD","resolution":null},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Proline utilization A","short_name":"PutA","sequence":"MGSSHHHHHHSSGLVPRGSH\r\nMGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSY\r\nLEQLENSDTLPEH\r\nMTDLSALGPKAKFAPEAEVLQALVAQAALPQPQLDRIAARGADLVAR\r\nIRAEAKPSLMEHFLAQYGLSTREGVALMCLAEAMLRVPDTATIDALIEDKIAPSDWGKHL\r\nGTAASSLVNASTWALMLTGKVLDDGAGGIAGTLRGAMRRLGEPVIRAAVGQAMREMGRQF\r\nVLGETIEKALERAEKREAEGYTFSYDMLGEAALTAADAERYRLAYAQAITAIGKAATRGS\r\nIAANPGISIKLSALHPRYEVAQEARVMAELVPVVRDLARAAARAGIALHIDAEEQDRLAL\r\nSLRVMAAVIADPETAGWEGFGAVVQAYGKRAGAAIDALAAMARAAGRRINIRLVKGAYWD\r\nAEMKRAQVEGHPGFPLFTSKTGTDVAYICLAAKLFGLNDCIYPQFATHNAHTVAAVLEMA\r\nAGRPFEFQRLHGMGARLHDIVLRETGGRCRIYAPVGAHRDLLAYLVRRLLENGANSSFVN\r\nQIVNESVPPAEVAACPFAALPTARAPRGLLAPADLFGAGRVNAQGFDLSDPEVLARIEAA\r\nRDVTLPDAAPIVAGPVSGTLRPVRNPATGAVVAQVTEADAATVALALDAAQVWSAPAATR\r\nAAVLCRAADLYEENFGPIFAALAQEAGKTLGDAVSELREAVDFLRYYAAEGAADTRPPRG\r\nAVVAISPWNFPLAIFTGQVAAALMAGNAVLAKPAEQTPIIAALAVRLLHQAGVPETALQL\r\nLPGDGPTVGAALTRDPRVAGVVFTGSTETAQIIARAMAAHLAPGTPLIAETGGLNAMVVD\r\nSTALPEQAVRDVVASAFRSAGQRCSALRCLYVQDDIAPHLIGMLKGAMEELVSGDPARLS\r\nTDVGPVIDAEAKAGIETYLAANKARILHRSTAPEGGHFVAPALLQVGGIADLEREIFGPV\r\nLHLATFAAEDLPAVIAAINARGYGLTFGLHSRIDARVETVAETIRAGNIYVNRNQIGAVV\r\nGSQPFGGEGLSGTGPKAGGPLYLNRFYAPEPVVAVGGWTEAATPILPEARETQLDEIFLP\r\nGPTGELNRLTRHQRGPILCLGPGAEAASAQAAAVVALGGQAVQASGAVSPKALETLTPLA\r\nGVLWWGAAEMGRAYAQALAVRPGPLVPLITAKPDLAHVAHERHLCVDTTAAGGNAALLAG","organism":"Escherchia coli, Rhodobacter capsulatus","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":null,"mw":125.528,"total_mw":251.056,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Tris, 200 mM NaCl, 0.5 mM Tris(3-hydroxypropyl)phosphine","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Chimeric EcRHH-RcPutA: The E.coli Proline utilization A RHH domain fused to R.capsulatus PutA","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"Mizzou","address":"Columbia, MO, USA","full_name":"University of Missouri-Columbia","webpage":"http://missouri.edu/"}],"contributor_name":"John","contributor_surname":"Tanner","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2012-10-16","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":0.5,"number_of_frames":2,"wavelength":0.1,"sample_detector_distance":1.5,"concentration_min":null,"concentration_max":6.0,"sample_volume":null,"flow_rate":null,"s_min":0.112,"s_max":3.165,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"GNOM","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of a chimeric EcRHH-RcPutA protein fusion in 50 mM Tris, 200 mM NaCl, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH 7.5 were collected on the 12.3.1 (SIBYLS) camera on the storage ring Advanced Light Source (ALS) (Berkeley, CA, USA) using a MAR 165 CCD detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and  2θ is the scattering angle). A solute concentrations of 6 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The merged SAXS curve displayed in this entry was obtained by merging the low-s region (points 2-140, s=0.1125 — 0.9517 nm-1) from an image collected with an exposure time of 0.5 s with the higher-s region (points 19-505, s=0.2159 — 3.1710 nm-1) of a profile collected with exposure time of 2.0 s. The errors in PDDF Rg and I0 were estimated by running GNOM via primus/qt (r4556) assuming Dmax in the range of 173-193 and using all points of the scattering curve (1-503).","tags":[],"intensity_unit":null,"experimental_mw":251.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":3033.0,"pddf_i0_error":5.5,"guinier_i0":3011.44,"guinier_i0_error":11.0,"pddf_rg":5.41,"pddf_rg_error":0.008,"guinier_rg":5.2,"guinier_rg_error":0.02,"pddf_dmax":18.3,"pddf_dmax_error":1.0,"porod_volume":308.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":23,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDB27_datloglog_img.png","symmetry":null,"last_modified":"2026-05-18T10:07:55.648439+02:00","bragg_peak":[]}