{"code":"SASDB28","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Structure and activation of C1, the complex initiating the classical pathway of the complement cascade.","publication":{"title":"Structure and activation of C1, the complex initiating the classical pathway of the complement cascade.","author_list":"Mortensen SA, Sander B, Jensen RK, Pedersen JS, Golas MM, Jensenius JC, Hansen AG, Thiel S, Andersen GR","journal":"Proc Natl Acad Sci U S A","doi":"10.1073/pnas.1616998114","pmid":"28104818","published_date":"2017 Jan 31"},"status":"released","submitted_date":"2016-12-02","released_date":"2017-01-09"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDB28.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB28_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB28_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB28_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDB28.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Complement C1q subcomponent subunit C","short_name":"C1qc","sequence":"EDLCRAPDGKKGEAGRPGRRGRPGLKGEQGEPGAPGIRTGIQGLKGDQGEPGPSGNPGKV\r\nGYPGPSGPLGARGIPGIKGTKGSPGNIKDQPRPAFSAIRRNPPMGGNVVIFDTVITNQEE\r\nPYQNHSGRFVCTVPGYYYFTFQVLSQWEICLSIVSSSRGQVRRSLGFCDTTNKGLFQVVS\r\nGGMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFSGFLIFPSA","organism":"Homo sapiens","uniprot_code":"P02747","uniprot_range_first":23,"uniprot_range_last":245,"oligomerization":"hexamer","molecular_type":"protein","uniprot_sequence":"MDVGPSSLPHLGLKLLLLLLLLPLRGQANTGCYGIPGMPGLPGAPGKDGYDGLPGPKGEP\nGIPAIPGIRGPKGQKGEPGLPGHPGKNGPMGPPGMPGVPGPMGIPGEPGEEGRYKQKFQS\nVFTVTRQTHQPPAPNSLIRFNAVLTNPQGDYDTSTGKFTCKVPGLYYFVYHASHTANLCV\nLLYRSGVKVVTFCGHTSKTNQVNSGGVLLRLQVGEEVWLAVNDYYDMVGIQGSDSVFSGF\nLLFPD","mw":23.687,"total_mw":142.122,"number_molecules":6,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Complement C1q subcomponent subunit B","short_name":"C1qb","sequence":"QLSCTGPPAIPGIPGIPGTPGPDGQPGTPGIKGEKGLPGLAGDHGEFGEKGDPGIPGNPG\r\nKVGPKGPMGPKGGPGAPGAPGPKGESGDYKATQKIAFSATRTINVPLRRDQTIRFDHVIT\r\nNMNNNYEPRSGKFTCKVPGLYYFTYHASSRGNLCVNLMRGRERAQKVVTFCDYAYNTFQV\r\nTTGGMVLKLEQGENVFLQATDKNSLLGMEGANSIFSGFLLFPDMEA","organism":"Homo sapiens","uniprot_code":"P02746","uniprot_range_first":28,"uniprot_range_last":253,"oligomerization":"hexamer","molecular_type":"protein","uniprot_sequence":"MMMKIPWGSIPVLMLLLLLGLIDISQAQLSCTGPPAIPGIPGIPGTPGPDGQPGTPGIKG\nEKGLPGLAGDHGEFGEKGDPGIPGNPGKVGPKGPMGPKGGPGAPGAPGPKGESGDYKATQ\nKIAFSATRTINVPLRRDQTIRFDHVITNMNNNYEPRSGKFTCKVPGLYYFTYHASSRGNL\nCVNLMRGRERAQKVVTFCDYAYNTFQVTTGGMVLKLEQGENVFLQATDKNSLLGMEGANS\nIFSGFLLFPDMEA","mw":23.742,"total_mw":142.452,"number_molecules":6,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Complement C1q subcomponent subunit A","short_name":"C1qa","sequence":"EDLCRAPDGKKGEAGRPGRRGRPGLKGEQGEPGAPGIRTGIQGLKGDQGEPGPSGNPGKV\r\nGYPGPSGPLGARGIPGIKGTKGSPGNIKDQPRPAFSAIRRNPPMGGNVVIFDTVITNQEE\r\nPYQNHSGRFVCTVPGYYYFTFQVLSQWEICLSIVSSSRGQVRRSLGFCDTTNKGLFQVVS\r\nGGMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFSGFLIFPSA","organism":"Homo sapiens","uniprot_code":"P02745","uniprot_range_first":23,"uniprot_range_last":245,"oligomerization":"hexamer","molecular_type":"protein","uniprot_sequence":"MEGPRGWLVLCVLAISLASMVTEDLCRAPDGKKGEAGRPGRRGRPGLKGEQGEPGAPGIR\nTGIQGLKGDQGEPGPSGNPGKVGYPGPSGPLGARGIPGIKGTKGSPGNIKDQPRPAFSAI\nRRNPPMGGNVVIFDTVITNQEEPYQNHSGRFVCTVPGYYYFTFQVLSQWEICLSIVSSSR\nGQVRRSLGFCDTTNKGLFQVVSGGMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFSGFL\nIFPSA","mw":23.687,"total_mw":142.122,"number_molecules":6,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM TrisHCl, 145 mM NaCl, 3 mM CaCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Complement factor 1q (C1q)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Aarhus, Denmark","full_name":"Aarhus University","webpage":"http://www.au.dk"}],"contributor_name":"Rasmus","contributor_surname":"Jensen","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2014-12-08","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":2.0,"number_of_frames":10,"wavelength":0.0992,"sample_detector_distance":null,"concentration_min":null,"concentration_max":3.66,"sample_volume":null,"flow_rate":null,"s_min":0.036,"s_max":4.975,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Complement factor 1q (C1q) in 50 mM TrisHCl, 145 mM NaCl, 3 mM CaCl2, pH 7.4, were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 3.7 and 3.7 mg/ml were measured at 4°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.","tags":[],"intensity_unit":null,"experimental_mw":407.0,"experimental_mw_error":null,"guinier_i0_mw":407.0,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":534.52,"guinier_i0_error":0.97,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":12.61,"guinier_rg_error":0.17,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":4,"guinier_point_last":15,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDB28_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}