{"code":"SASDB38","status":"Published","type_of_curve":"Other","angular_unit":"1/nm","project":{"title":"Structure and activation of C1, the complex initiating the classical pathway of the complement cascade.","publication":{"title":"Structure and activation of C1, the complex initiating the classical pathway of the complement cascade.","author_list":"Mortensen SA, Sander B, Jensen RK, Pedersen JS, Golas MM, Jensenius JC, Hansen AG, Thiel S, Andersen GR","journal":"Proc Natl Acad Sci U S A","doi":"10.1073/pnas.1616998114","pmid":"28104818","published_date":"2017 Jan 31"},"status":"released","submitted_date":"2016-12-02","released_date":"2017-01-09"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDB38.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDB38.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB38_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB38_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB38_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB38_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDB38.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray 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C1s subcomponent","short_name":"C1s","sequence":"EPTMYGEILSPNYPQAYPSEVEKSWDIEVPEGYGIHLYFTHLDIELSENCAYDSVQIISG\r\nDTEEGRLCGQRSSNNPHSPIVEEFQVPYNKLQVIFKSDFSNEERFTGFAAYYVATDINEC\r\nTDFVDVPCSHFCNNFIGGYFCSCPPEYFLHDDMKNCGVNCSGDVFTALIGEIASPNYPKP\r\nYPENSRCEYQIRLEKGFQVVVTLRREDFDVEAADSAGNCLDSLVFVAGDRQFGPYCGHGF\r\nPGPLNIETKSNALDIIFQTDLTGQKKGWKLRYHGDPMPCPKEDTPNSVWEPAKAKYVFRD\r\nVVQITCLDGFEVVEGRVGATSFYSTCQSNGKWSNSKLKCQPVDCGIPESIENGKVEDPES\r\nTLFGSVIRYTCEEPYYYMENGGGGEYHCAGNGSWVNEVLGPELPKCVPVCGVPREPFEEK\r\nQRIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGNREPTMYVGSTSVQTS\r\nRLAKSKMLTPEHVFIHPGWKLLEVPEGRTNFDNDIALVRLKDPVKMGPTVSPICLPGTSS\r\nDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVKVEKPTADAEAYVFTPN\r\nMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCGTYGLYTRVKNYVDWI\r\nMKTMQENSTPRED","organism":"Homo sapiens","uniprot_code":"P09871","uniprot_range_first":16,"uniprot_range_last":688,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MWCIVLFSLLAWVYAEPTMYGEILSPNYPQAYPSEVEKSWDIEVPEGYGIHLYFTHLDIE\nLSENCAYDSVQIISGDTEEGRLCGQRSSNNPHSPIVEEFQVPYNKLQVIFKSDFSNEERF\nTGFAAYYVATDINECTDFVDVPCSHFCNNFIGGYFCSCPPEYFLHDDMKNCGVNCSGDVF\nTALIGEIASPNYPKPYPENSRCEYQIRLEKGFQVVVTLRREDFDVEAADSAGNCLDSLVF\nVAGDRQFGPYCGHGFPGPLNIETKSNALDIIFQTDLTGQKKGWKLRYHGDPMPCPKEDTP\nNSVWEPAKAKYVFRDVVQITCLDGFEVVEGRVGATSFYSTCQSNGKWSNSKLKCQPVDCG\nIPESIENGKVEDPESTLFGSVIRYTCEEPYYYMENGGGGEYHCAGNGSWVNEVLGPELPK\nCVPVCGVPREPFEEKQRIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGN\nREPTMYVGSTSVQTSRLAKSKMLTPEHVFIHPGWKLLEVPEGRTNFDNDIALVRLKDPVK\nMGPTVSPICLPGTSSDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVKV\nEKPTADAEAYVFTPNMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCG\nTYGLYTRVKNYVDWIMKTMQENSTPRED","mw":74.886,"total_mw":149.772,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM EPPS, 145 mM NaCl, 3 mM CaCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.5,"deuteration":null},"purity_method":null,"name":"Inactivated complement factor 1 (C1)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Aarhus, Denmark","full_name":"Aarhus University","webpage":"http://www.au.dk"}],"contributor_name":"Rasmus","contributor_surname":"Jensen","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-08-16","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":100,"wavelength":0.124,"sample_detector_distance":null,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.043,"s_max":2.531,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDB38_fit1_model1_img.png","software":"CORAL","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"CORALXL","model_data":"https://www.sasbdb.org/media/pdb_file/SASDB38_fit1_model1.pdb","model_mw":699.6,"bead_radius":1.9,"log":null,"symmetry":"P2","comment":null,"user":185}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB38_fit1_fixed_fit_img.png","software":"CORAL","chi_square_value":2.34,"p_value":0.3671,"fit_residual_plot":"SASDB38_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDB38_fit1.fit","fit_log":null,"software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDB38_fit2_model1_img.png","software":"CORAL","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"CORALXL","model_data":"https://www.sasbdb.org/media/pdb_file/SASDB38_fit2_model1.pdb","model_mw":699.6,"bead_radius":1.9,"log":null,"symmetry":"P2","comment":null,"user":185}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB38_fit2_fixed_fit_img.png","software":"CORAL","chi_square_value":2.44,"p_value":0.2038,"fit_residual_plot":"SASDB38_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDB38_fit2.fit","fit_log":null,"software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDB38_fit3_model1_img.png","software":"CORAL","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"CORALXL","model_data":"https://www.sasbdb.org/media/pdb_file/SASDB38_fit3_model1.pdb","model_mw":699.6,"bead_radius":1.9,"log":null,"symmetry":"P2","comment":null,"user":185}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB38_fit3_fixed_fit_img.png","software":"CORAL","chi_square_value":2.3,"p_value":0.9768,"fit_residual_plot":"SASDB38_fit3_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDB38_fit3.fit","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5","i0_calibration_standard":null,"description":"The expected MW quoted above (733 kDa) is calculated from the amino acid sequence of the oligomeric complex. The experimental MW of 790 kDa takes into account additional post-translational modifications of the protein. The models displayed in this entry - sequentially from top to bottom - represent those presented in Figure 4c of the main text and Supplementary Figures 2e and 4b, respectively, of Mortensen et al., 2017 Proc Natl Acad Sci U S A 114(5):986-991 .","experiment_description":"Synchrotron SAXS data from solutions of Inactivated complement factor 1 (C1) in 50 mM EPPS, 145 mM NaCl, 3 mM CaCl2, pH 8.5, were collected using size-exclusion chromatography (SEC) SAXS on the EMBL-P12 bioSAXS beam line at the PETRAIII storage ring (Hamburg, Germany) equipped with a Pilatus 2M detector (I(s) vs s, where s = 4π sin θ/λ; 2θ is the scattering angle; λ = 0.124 nm). 100 successive 1 second frames were collected through the C1 SEC elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank (measured from macromolecular-free SEC running buffer) was subtracted and the resulting 1D profiles were scaled relative to each other and averaged to produce the final C1 SAXS data displayed in this entry.","tags":[],"intensity_unit":null,"experimental_mw":790.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":49450.0,"pddf_i0_error":37.0,"guinier_i0":49602.1,"guinier_i0_error":80.17,"pddf_rg":11.46,"pddf_rg_error":0.01,"guinier_rg":11.5,"guinier_rg_error":0.1,"pddf_dmax":36.6,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":27,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDB38_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}