{"code":"SASDB54","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.","publication":{"title":"Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.","author_list":"Batista FAH, Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC","journal":"Arch Biochem Biophys","doi":"10.1016/j.abb.2016.04.008","pmid":"27103305","published_date":"2016 Jun 15"},"status":"released","submitted_date":"2016-02-21","released_date":"2016-08-01"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDB54.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDB54.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB54_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB54_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB54_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB54_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDB54.sascif","experiment":{"instrument":{"detector":{"type":"20Hz","name":"Pilatus 300K","resolution":null},"name":"Brazilian Synchrotron Light Laboratory","city":"Campinas","country":"Brazil","beamline_name":"SAXS1 Beamline","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Stress-induced protein sti1","short_name":"Hop/Sti1","sequence":"MDANELKNEGNKEFSAGRYVEAVNYFSKAIQLDGQNSVLYSNRSACFAAMQKYKDALDDADKCISIKPNW\r\nAKGYVRRGAALHGMRRYDDAIAAYEKGLSVDPSNSGCTQGVKDVQVAKSREARDPIARVFTPEAFRKIQE\r\nNPKLSLLMLQPDYVKMVDTVVRDPSQARLYMEDQRFALTLMYLSGMKIPNDDEDDEEERPSAKAAAEAKA\r\nKEEKKLLTDNEKEAMALKEEGNKLYLSKRFEEALSKYQEAQAKDPKNTLYILNVSAVYFEQRDYEKCITE\r\nCERGIEHGRENHCDYTIVAKLMTRHAFCLQKQKKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVE\r\nEAYIDPEIARQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCI\r\nELKPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRLRTIMRIQEMASGQSADGDEAAR\r\nRAMDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDPGISVKINKLISAGIIRFGQ","organism":"Leishmania braziliensis","uniprot_code":"A4H5F0","uniprot_range_first":1,"uniprot_range_last":547,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MDANELKNEGNKEFSAGRYVEAVNYFSKAIQLDGQNSVLYSNRSACFAAMQKYKDALDDA\nDKCISIKPNWAKGYVRRGAALHGMRRYDDAIAAYEKGLSVDPSNSGCTQGVKDVQVAKSR\nEARDPIARVFTPEAFRKIQENPKLSLLMLQPDYVKMVDTVVRDPSQARLYMEDQRFALTL\nMYLSGMKIPNDDEDDEEERPSAKAAAEAKAKEEKKLLTDNEKEAMALKEEGNKLYLSKRF\nEEALSKYQEAQAKDPKNTLYILNVSAVYFEQRDYEKCITECERGIEHGRENHCDYTIVAK\nLMTRHAFCLQKQKKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVEEAYIDPEIAR\nQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCI\nELKPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRLRTIMRIQEMASG\nQSADGDEAARRAMDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDPGISVKINKLISA\nGIIRFGQ","mw":62.452,"total_mw":62.452,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol","concentration_unit":"mM","comment":null,"additive":"100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol","concentration":25.0,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Leishmania braziliensis stress-induced protein sti1 (LbHop), full length construct","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Júlio","contributor_surname":"Borges","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2016-02-21","storage_temperature":null,"cell_temperature":null,"exposure_time":null,"number_of_frames":null,"wavelength":null,"sample_detector_distance":null,"concentration_min":0.8,"concentration_max":1.8,"sample_volume":null,"flow_rate":null,"s_min":0.123,"s_max":4.067,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDB54_fit1_model1_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDB54_fit1_model1.pdb","model_mw":58.95,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":100}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB54_fit1_fit_img.png","software":null,"chi_square_value":3.549,"p_value":0.0,"fit_residual_plot":"SASDB54_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDB54_fit1.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from solutions of stress-induced protein sti1 (Hop/Sti1) in 25 mM Tris 100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol were collected on the SAXS1 and SAXS2 beam lines at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a MAR Image Plate and Pilatus 300k detectors (s = 4π sin θ/λ, where 2θ is the scattering angle). Different solute concentrations in the range 0.80-1.80 mg/ml were measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were merged to yield the final composite scattering curve. ","tags":[],"intensity_unit":null,"experimental_mw":63.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":59.0,"porod_mw_error":null,"pddf_i0":0.0632,"pddf_i0_error":0.0002,"guinier_i0":0.063,"guinier_i0_error":null,"pddf_rg":4.68,"pddf_rg_error":0.02,"guinier_rg":4.51,"guinier_rg_error":0.05,"pddf_dmax":18.0,"pddf_dmax_error":null,"porod_volume":94.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":3,"guinier_point_last":16,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDB54_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}