{"code":"SASDB65","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Global Disordering in Stereo-Specific Protein Association","publication":{"title":"Global Disordering in Stereo-Specific Protein Association","author_list":"Gupta A, Reinartz I, Spilotros A, Jonna V, Hofer A, Svergun D, Schug A, Wolf-Watz M","journal":"Biophysical Journal","doi":"10.1016/j.bpj.2016.11.211","pmid":null,"published_date":"2017 Feb"},"status":"released","submitted_date":"2016-08-12","released_date":"2017-04-21"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDB65.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDB65.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB65_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB65_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB65_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB65_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDB65.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"SycH putative yopH targeting protein","short_name":"SycH","sequence":"MRTYSSLLEEFATELGLEEIETNELGHGAVTIDKIWVVHLAPINEKELVAFMRAGILTGQSQLYDILRKNLFSPLSGVIRCALDKDDHWLLWSQLNINDTSGTQLASVLTSLVDKAVTLSCEPTMKKEEDDHRPSSSHLLV","organism":"Yersinia pseudotuberculosis","uniprot_code":"I3NIC8","uniprot_range_first":1,"uniprot_range_last":141,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MRTYSSLLEEFATELGLEEIETNELGHGAVTIDKIWVVHLAPINEKELVAFMRAGILTGQ\nSQLYDILRKNLFSPLSGVIRCALDKDDHWLLWSQLNINDTSGTQLASVLTSLVDKAVTLS\nCEPTMKKEEDDHRPSSSHLLV","mw":15.763,"total_mw":31.526,"number_molecules":2,"complex_state":null,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Tyrosine-protein phosphatase YopH","short_name":"YopH","sequence":"MNLSLSDLHRQVSRLVQQESGDCTGKLRGNVAANKETTFQGLTIASGARESEKVFAQTVLSHVANVVLTQEDTAKLLQST\r\nVKHNLNNYDLRSVGNGNSVLVSLRSDQMTLQDAKVLLEAALRQESGARG","organism":"Yersinia pseudotuberculosis","uniprot_code":"P08538","uniprot_range_first":1,"uniprot_range_last":129,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MNLSLSDLHRQVSRLVQQESGDCTGKLRGNVAANKETTFQGLTIASGARESEKVFAQTVL\nSHVANVVLTQEDTAKLLQSTVKHNLNNYDLRSVGNGNSVLVSLRSDQMTLQDAKVLLEAA\nLRQESGARGHVSSHSHSALHAPGTPVREGLRSHLDPRTPPLPPRERPHTSGHHGAGEARA\nTAPSTVSPYGPEARAELSSRLTTLRNTLAPATNDPRYLQACGGEKLNRFRDIQCCRQTAV\nRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAENRTPVLAVLASSSEIANQRFGMPD\nYFRQSGTYGSITVESKMTQQVGLGDGIMADMYTLTIREAGQKTISVPVVHVGNWPDQTAV\nSSEVTKALASLVDQTAETKRNMYESKGSSAVGDDSKLRPVIHCRAGVGRTAQLIGAMCMN\nDSRNSQLSVEDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQGRPLLNS","mw":13.95,"total_mw":13.95,"number_molecules":1,"complex_state":null,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM HEPES 2mM TCEP","concentration_unit":"mM","comment":null,"additive":"2mM TCEP","concentration":50.0,"pka":null,"ph":6.8,"deuteration":null},"purity_method":null,"name":"Tyrosine-protein phosphatase (YopH)/putative yopH targeting protein (SycH) heterotrimeric complex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Alessandro","contributor_surname":"Spilotros","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2016-08-01","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.12,"sample_detector_distance":3.1,"concentration_min":null,"concentration_max":1.0,"sample_volume":null,"flow_rate":null,"s_min":0.066,"s_max":4.808,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDB65_fit1_model1_img.png","software":"CRYSOL","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDB65_fit1_model1.pdb","model_mw":45.45,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":109}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB65_fit1_fit_img.png","software":null,"chi_square_value":1.27,"p_value":0.0,"fit_residual_plot":"fit4columns464_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDB65_fit1.fit","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from a solution of dimeric YopH protein in complex with the tyrosine-protein phosphatase, SycH, in 50 mM HEPES, 2 mM TCEP, pH 6.8 were collected on the P12 beam line of Petra-III (Hamburg, Germany) using a Pilatus 2M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.12 nm). One solute concentration of 1.00 mg/ml was measured using an exposure time of 1 s (recorded as 20 x 0.050 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were scaled to protein concentration. The results presented here were obtained from a single concentration scattering curve.","tags":[],"intensity_unit":null,"experimental_mw":52.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":55.8,"porod_mw_error":null,"pddf_i0":74550.0,"pddf_i0_error":130.0,"guinier_i0":74475.0,"guinier_i0_error":117.0,"pddf_rg":3.01,"pddf_rg_error":0.013,"guinier_rg":2.96,"guinier_rg_error":0.13,"pddf_dmax":12.5,"pddf_dmax_error":null,"porod_volume":89.2,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":22,"guinier_point_last":115,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDB65_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}