{"code":"SASDB68","status":"Published","type_of_curve":"Other","angular_unit":"1/A","project":{"title":"The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.","publication":{"title":"The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.","author_list":"Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkx419","pmid":"28505309","published_date":"2017 Jul 27"},"status":"released","submitted_date":"2016-11-28","released_date":"2017-05-30"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDB68.dat","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDB68.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB68_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB68_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB68_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB68_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDB68.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Immediate Early 3","short_name":"IE3","sequence":"CCGATCGTCCACACGGAGC","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"DNA","uniprot_sequence":null,"mw":5.839,"total_mw":11.678,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES, 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Immediate Early 3 DNA (ICP4N IE3_19mer)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Manchester, UK","full_name":"University of Manchester","webpage":"http://www.manchester.ac.uk/"}],"contributor_name":"Michael","contributor_surname":"Lockhart","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-11-12","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":null,"wavelength":0.0992,"sample_detector_distance":2.9,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.224,"s_max":3.396,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ScÅtter","pddf_software_version":"3.0g","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Immediate Early 3 DNA (ICP4N IE3_19mer) in 20 mM HEPES, 150 mM NaCl, pH 7.4, were collected using HPLC SEC-SAXS at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The sample was separated using a Superdex 200 increase 3.2/300 size exclusion chromatography column before exposure to X-rays. 50 µL of IE3_19merwas loaded onto the Superdex column and the eluent was flowed through the SAXS beam at 0.075 mL/min; the buffer used as the background was collected after one SEC column volume. Data were collected at 20°C at one second intervals.","tags":[],"intensity_unit":null,"experimental_mw":0.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":44.35,"pddf_i0_error":null,"guinier_i0":47.86,"guinier_i0_error":0.048,"pddf_rg":1.925,"pddf_rg_error":null,"guinier_rg":1.93,"guinier_rg_error":0.011,"pddf_dmax":6.9,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":34,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDB68_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}