{"code":"SASDB69","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Functional and structural insight into properdin control of complement alternative pathway amplification.","publication":{"title":"Functional and structural insight into properdin control of complement alternative pathway amplification.","author_list":"Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR","journal":"EMBO J","doi":"10.15252/embj.201696173","pmid":"28264884","published_date":"2017 Apr 13"},"status":"released","submitted_date":"2017-01-02","released_date":"2017-03-12"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDB69.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB69_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB69_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB69_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDB69.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"E244K Human Properdin","short_name":"E244K FP","sequence":"DPVLCFTQYEESSGKCKGLLGGGVSVEDCCLNTAFAYQKRSGGLCQPCRSPRWSLWSTWA\r\nPCSVTCSEGSQLRYRRCVGWNGQCSGKVAPGTLEWQLQACEDQQCCPEMGGWSGWGPWEP\r\nCSVTCSKGTRTRRRACNHPAPKCGGHCPGQAQESEACDTQQVCPTHGAWATWGPWTPCSA\r\nSCHGGPHEPKETRSRKCSAPEPSQKPPGKPCPGLAYKQRRCTGLPPCPENLYFQGVAGGW\r\nGPWGPVSPCPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDATRTHICNTAVPCPVDGEWDS\r\nWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRCAGQQQDIRHCYSIQHCPLKG\r\nSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGRPLPRCE\r\nELQGQKLVVEEKRPCLHVPACKDPEEEEL","organism":"Homo sapiens","uniprot_code":"P27918","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MITEGAQAPRLLLPPLLLLLTLPATGSDPVLCFTQYEESSGKCKGLLGGGVSVEDCCLNT\nAFAYQKRSGGLCQPCRSPRWSLWSTWAPCSVTCSEGSQLRYRRCVGWNGQCSGKVAPGTL\nEWQLQACEDQQCCPEMGGWSGWGPWEPCSVTCSKGTRTRRRACNHPAPKCGGHCPGQAQE\nSEACDTQQVCPTHGAWATWGPWTPCSASCHGGPHEPKETRSRKCSAPEPSQKPPGKPCPG\nLAYEQRRCTGLPPCPVAGGWGPWGPVSPCPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDA\nTRTHICNTAVPCPVDGEWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRC\nAGQQQDIRHCYSIQHCPLKGSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSM\nVEGQGEKNVTFWGRPLPRCEELQGQKLVVEEKRPCLHVPACKDPEEEEL","mw":49.345,"total_mw":49.345,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"10 mM HEPES 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.2,"deuteration":null},"purity_method":null,"name":"E244K monomeric human Properdin","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Aarhus, Denmark","full_name":"Aarhus University","webpage":"http://www.au.dk"}],"contributor_name":"Rasmus","contributor_surname":"Jensen","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-07-27","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":2.7,"sample_volume":null,"flow_rate":null,"s_min":0.154,"s_max":2.799,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDB69_fit1_model1_img.png","software":"CORAL","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDB69_fit1_model1.pdb","model_mw":53.64,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":185}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB69_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":1.61,"p_value":0.0,"fit_residual_plot":"SASDB69_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDB69_fit1.fit","fit_log":null,"software_version":"Crysol3","description":""}],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of recombinantly formed monomeric human E244K properdin in 10 mM HEPES pH 7.2, 150 mM NaCl, were collected EMBL-P12 bioSAXS beam line at the PETRAIII storage ring (Hamburg, Germany) equipped with a Pilatus 2M detector (I(s) vs s, where s = 4π sin θ/λ; 2θ is the scattering angle; λ = 0.124 nm). A solute concentration of 2.7 mg/ml was measured at 20°C. 20 successive 0.05 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and scaled for protein concentration.","tags":[],"intensity_unit":null,"experimental_mw":50.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":16659.0,"guinier_i0_error":67.36,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":4.13,"guinier_rg_error":0.04,"pddf_dmax":15.0,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":59,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDB69_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}