{"code":"SASDB85","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Self-processing module of Iron-regulated protein FrpC","publication":null,"status":"released","submitted_date":"2016-08-16","released_date":"2021-09-13"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDB85.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDB85.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB85_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB85_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB85_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB85_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDB85.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"CEITEC","city":"Brno","country":"Czech Republic","beamline_name":"Rigaku BioSAXS-1000","beam_geometry":"","type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Iron-regulated protein FrpC (amino acids 415-591)","short_name":"FrpC:415-591","sequence":"PLALDLDGDGIETVATKGFSGSLFDHNRDGIRTATGWVSADDGLLVRDLNGNGIIDNGAELF\r\nGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQSLRVWQDLNQDGISQANELRTL\r\nEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTNGTTAKMGDLLLAADNLHSRFLE","organism":"Neisseria meningitidis","uniprot_code":"Q9JYV5","uniprot_range_first":415,"uniprot_range_last":591,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAAAIVGKDANLNGLNLWM\nKKGVENLWDDTVGKKTRLEKFDRVALQHFRQYARLINQNNGRLPNTSEIERSYYKAVTDN\nGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQAVNNPNGSERDNRKQLISALD\nKGFDGSFKEKHFTFLQSVMMDVTKLGVEYTIDGWQKIGGWGNGIINDLYKSVVKREWTGI\nFEIVNNNIKQGNEAFKNEINSLVHDMKAAGKEFGDDLNTQWNNLTQAAEIIYNDIVDNTS\nQGIEKGVKAIKELSEKMKNAASDLADGSAEKAKQVVEDLAQAAKEAYENAKSTAEKAAQA\nAREFFKGLPSFKDLAEKFRDLFPNPEGWIDDGHQCLAPWVKETKKRNGKYHVYDPLALDL\nDGDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVRDLNGNGIIDNGAELFGDNT\nKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVWQDLNQDGISQANELRTLEELG\nIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKMGDLLLAADNLHSRFKDKVELTAE\nQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSNW\nGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKAAIDAARDRIAVLDAYT\nGQDSNTLYYMSEEDALNIVKVTNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKMENDTFT\nLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMTDYVEEAKKAGKFEDY\nQKVLGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYGNDGNDTLIGGAGNDYLEGGSG\nSDTYVFGEGFGQDTVYNYDYATGRKDIIRFTDGITADMLTFTREGNHLLIKAKDGSGQVT\nVQSYFQNDGSGAYRIDEIHFDNGKVLDVATVKELVQQSTDGSDRLYAYQSGNTLNGGLGD\nDYLYGADGDDLLNGDAGNDSIYSGNGNDTLDGGEGNDALYGYNGNDALNGGEGNDHLNGE\nDGNDTLIGGAGNDYLEGGSGSDTYVFGKGFGQDTVYNYDYATGRKDIIRFTDGITADMLT\nFTREGNHLLIKAKDGSGQVTVQYYFQNDGSGAYRIDEIHFDNGKVLDVATVKELVQQSTD\nGSDRLYAYQSGNTLNGGLGDDYLYGADGDDLLNGDAGNDSIYSGNGNDTLDGGEGNDALY\nGYNGNDALNGGEGNDHLNGEDGNDTLIGGAGNDYLEGGSGSDTYVFGKGFGQDAVYNYDY\nATGRKDIIRFTDGITADMLTFTREGNHLLIKAKDGSGQVTVQSYFQNDGSGAYRIDEIHF\nDNGKVLDVATVKELVQQSTDGSDRLYAYQSGNTLNGGLGDDYLYGADGDDLLNGDAGNDS\nIYSGNGNDTLNGGEGNDALYGYNGNDALNGGEGNDHLNGEDGNDTLIGGAGNDYLEGGSG\nSDTYVFGKGFGQDAVYNYDYATGRKDIIRFTDGITADMLTFTREGNHLLIKAKDGSGQVT\nVQSYFQNDGSGAYRIDEIHFDNGKVLDVATVKELVQQSTDGSDRLYAYQSGSTLNGGLGD\nDYLYGADGDDLLNGDAGNDSIYSGNGNDTLDGGEGNDALYGYNGNDALNGGEGNDHLNGE\nDGNDTLIGGAGNDYLEGGSGSDTYVFGEGFGQDTVYNYHVDKNSDTMHFKGFKAADVHFI\nRSGSDLVLSASEQDNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSV\nFGSNTAATGGNVDANIQSVQQPLLVTPSA","mw":18.698,"total_mw":18.698,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"5 mM Tris 50 mM NaCl 10 mM CaCl2 0.1% NaN3","concentration_unit":"mM","comment":null,"additive":"50 mM NaCl, 10 mM CaCl2, 0.1% NaN3","concentration":5.0,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Self-processing module of iron-regulated protein FrpC (415-591)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"MUNI","address":"Brno, Czech Republic","full_name":"Masaryk University;   \tResearch group Structure and Dynamics of Proteins.","webpage":"https://www.muni.cz/"}],"contributor_name":"Vojtěch","contributor_surname":"Kubáň","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2013-10-03","storage_temperature":30.0,"cell_temperature":30.0,"exposure_time":600.0,"number_of_frames":4,"wavelength":0.1542,"sample_detector_distance":null,"concentration_min":1.25,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.08,"s_max":4.99,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDB85_fit1_model1_img.png","software":"Other","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDB85_fit1_model1.pdb","model_mw":382.8,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":146}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB85_fit1_fixed_fit_img.png","software":null,"chi_square_value":1.9419,"p_value":0.00062,"fit_residual_plot":"SASDB85_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDB85_fit1.fit","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"In-house small-angle X-ray scattering data from solutions of iron-regulated protein FrpC (amino acids 415-591) in 5 mM Tris 50 mM NaCl, 10 mM CaCl2, 0.1% NaN3, pH 7.4 were collected using a Rigaku BioSAXS-1000 SAXS instrument (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.154 nm). Different solute concentrations in the range 1.25-5.00 mg/ml were measured. Four successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were obtained from a single concentration scattering curve.","tags":[],"intensity_unit":null,"experimental_mw":18.4,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.1355,"pddf_i0_error":0.0005,"guinier_i0":0.14,"guinier_i0_error":0.0007,"pddf_rg":1.643,"pddf_rg_error":0.005,"guinier_rg":1.7,"guinier_rg_error":0.05,"pddf_dmax":4.51,"pddf_dmax_error":null,"porod_volume":20.5,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":16,"guinier_point_last":47,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDB85_datloglog_img.png","symmetry":null,"last_modified":"2021-09-13T16:54:31.604815+02:00","bragg_peak":[]}