{"code":"SASDBC4","status":"Published","type_of_curve":"Extrapolated to infinite dilution","angular_unit":"1/nm","project":{"title":"The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.","publication":{"title":"The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.","author_list":"Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM","journal":"J Biol Chem","doi":"10.1074/jbc.M116.732909","pmid":"27413182","published_date":"2016 Sep 2"},"status":"released","submitted_date":"2016-07-14","released_date":"2016-07-14"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBC4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBC4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBC4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBC4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBC4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBC4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBC4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray 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","mw":95.573,"total_mw":95.573,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT","concentration_unit":"mM","comment":null,"additive":"150 mM NaCl, 5% glycerol, 3 mM DTT","concentration":20.0,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Plakin domain of Human plectin (spectrin repeats: SR3-SR9)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"USAL","address":"Salamanca, Spain","full_name":"La Universidad de Salamanca","webpage":"http://www.usal.es"}],"contributor_name":"Jose M","contributor_surname":"de Pereda","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2013-08-13","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.12,"sample_detector_distance":3.1,"concentration_min":0.92,"concentration_max":14.7,"sample_volume":null,"flow_rate":null,"s_min":0.054,"s_max":3.495,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBC4_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBC4_fit1_model1.pdb","model_mw":94.0,"bead_radius":6.5,"log":null,"symmetry":"","comment":"","user":26}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBC4_fit1_fit_img.png","software":null,"chi_square_value":1.509,"p_value":9e-05,"fit_residual_plot":"SASDBC4_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBC4_fit1.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the plakin domain from Human plectin in 20 mM sodium phosphate, 150 mM NaCl, 5% v/v glycerol, 3 mM DTT were collected on the P12 beam line of Petra-III (Hamburg, Germany) using a Pilatus 2M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.124 nm). Different solute concentrations in the range 0.92-14.70 mg/ml were measured using an exposure time of 1 s (recorded as 20 x 0.050 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were normalized to protein concentration and then extrapolated to infinite dilution to simulate a zero-concentration scattering curve.","tags":[],"intensity_unit":null,"experimental_mw":93.2,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":84.6,"porod_mw_error":null,"pddf_i0":2818.0,"pddf_i0_error":null,"guinier_i0":2789.0,"guinier_i0_error":null,"pddf_rg":9.08,"pddf_rg_error":null,"guinier_rg":8.54,"guinier_rg_error":null,"pddf_dmax":35.0,"pddf_dmax_error":null,"porod_volume":135.3,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":null,"guinier_point_last":null,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBC4_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}