{"code":"SASDBC7","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.","publication":{"title":"Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.","author_list":"Prakash A, Moharana K, Wallace SS, Doublié S","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkw1282","pmid":"27994037","published_date":"2017 Mar 17"},"status":"released","submitted_date":"2016-11-04","released_date":"2017-01-03"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBC7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBC7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBC7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBC7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBC7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBC7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBC7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"MAR 165 CCD","resolution":null},"name":"Advanced Photon Source (APS), Argonne National Laboratory","city":"Lemont, IL","country":"USA","beamline_name":"BioCAT 18ID","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Endonuclease 8-like 1","short_name":null,"sequence":"MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRESVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSASLEHHHHHH","organism":"Homo sapiens","uniprot_code":"Q96FI4","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLIL\nSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRF\nGRWDLGGKWQPGRGPCVLQEYQQFRENVLRNLADKAFDRPICEALLDQRFFNGIGNYLRA\nEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLG\nGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKS\nKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRR\nKGRQAASGHCRPRKVKADIPSLEPEGTSAS","mw":44.722,"total_mw":44.722,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25mM HEPES 300mM NaCl 1mM DTT 10% Glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Human NEI like DNA glycosylase 1 (NEIL1)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Burlington, VT, USA","full_name":"University of Vermont","webpage":"https://www.uvm.edu/"}],"contributor_name":"Kedar","contributor_surname":"Moharana","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-03-13","storage_temperature":4.0,"cell_temperature":10.0,"exposure_time":1.0,"number_of_frames":14,"wavelength":0.1033,"sample_detector_distance":null,"concentration_min":0.5,"concentration_max":2.0,"sample_volume":null,"flow_rate":null,"s_min":0.05,"s_max":3.286,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBC7_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBC7_fit1_model1.pdb","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":177}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBC7_fit1_fit_img.png","software":null,"chi_square_value":1.07,"p_value":0.120471,"fit_residual_plot":"SASDBC7_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBC7_fit1.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"ATSAS GNOM 5.0","i0_calibration_standard":null,"description":"Experimental MW reported here is calculated from Gnom based I(0) estimates and will be used for publication purpose in future. Guinier I(0) estimates are prone to presence of aggregates/radiation damage and that is why Gnom based I(0) was preferred to calculate the experimental MW.","experiment_description":null,"tags":[],"intensity_unit":null,"experimental_mw":48.1,"experimental_mw_error":null,"guinier_i0_mw":50.56,"guinier_i0_mw_error":0.35,"porod_mw":47.8,"porod_mw_error":null,"pddf_i0":4.411,"pddf_i0_error":0.0441,"guinier_i0":4.31,"guinier_i0_error":0.03,"pddf_rg":3.83,"pddf_rg_error":0.064,"guinier_rg":3.55,"guinier_rg_error":0.331,"pddf_dmax":15.0,"pddf_dmax_error":0.25,"porod_volume":81.37,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":28,"guinier_point_last":107,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBC7_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}