{"code":"SASDBD3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.","publication":{"title":"Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.","author_list":"Seraphim TV, Silva KP, Dores-Silva PR, Barbosa LR, Borges JC","journal":"Int J Biol Macromol","doi":"10.1016/j.ijbiomac.2017.01.058","pmid":"28104372","published_date":"2017 Apr"},"status":"released","submitted_date":"2016-01-18","released_date":"2016-01-21"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBD3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBD3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBD3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBD3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBD3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBD3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBD3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"MAR 165 CCD","resolution":null},"name":"Brazilian Synchrotron Light Laboratory","city":"Campinas","country":"Brazil","beamline_name":"SAXS2 Beamline","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Leishmania braziliensis heat shock protein 90 (Hsp90)","short_name":"L. brazilien.Hsp90","sequence":"MGSSHHHHHHSSGLVPRGSHMTETFAFQAEINQLMSLIINTFYSNKEIFLRELISNASDACDKIRYQSLTDPSVLGEETH\r\nLRVRVVPDKANKTLTVEDNGIGMTKADLVNNLGTIARSGTKAFMEALEAGGDMSMIGQFGVGFYSAYLVADRVTVVSKNN\r\nSDEAYVWESSAGGTFTITSVPESDMKRGTRITLHLKEDQQEYLEERRVKELIKKHSEFIGYDIELMVEKTAEKEVTDEDE\r\nEEDESKKKSCGDEGEPKVEEVTEGGEDKKKKTKKVKEVTKTYEVQNKHKPLWTRDPKDVTKEEYAAFYKAISNDWEDPAA\r\nTKHFSVEGQLEFRAIAFVPKRAPFDMFEPNKKRNNIKLYVRRVFIMDNCEDLCPDWLGFVKGVVDSEDLPLNISRENLQQ\r\nNKILKVIRKNIVKKCLELFEEMAENKEDYKQFYEQFGKNIKLGIHEDTANRKKLMELLRFYSTESGEEMTTLKDYVTRMK\r\nPEQKSIYYITGDSKKKLESSPFIEKARRCGLEVLFMTEPIDEYVMQQVKDFEDKKFACLTKEGVHFEESEEEKKQREEKK\r\nAACEKLCKTMKEVLGDKVEKVTVSERLSTSPCILVTSEFGWSAHMEQIMRNQALRDSSMAQYMVSKKTMEVNPDHPIIKE\r\nLRRRVEADENDKAVKDLVFLLFDTSLLTSGFQLDDPTGYAERINRMIKLGLSLDEEDEEVAEAPPAEAAPAEVTAGTSSM\r\nEQVD","organism":"Leishmania braziliensis","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":null,"mw":82.8,"total_mw":165.6,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol","concentration_unit":"mM","comment":null,"additive":"100 mM NaCl, 1 mM 2-mercaptoethanol","concentration":25.0,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Leishmania braziliensis heat shock protein 90 (Hsp90).","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"São Paulo, SP, Brazil","full_name":"Instituto de Física, Universidade de São Paulo","webpage":""}],"contributor_name":"Leandro","contributor_surname":"Barbosa","orcid":null},{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Júlio","contributor_surname":"Borges","orcid":null},{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Paulo","contributor_surname":"Dores-Silva","orcid":null},{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Thiago","contributor_surname":"Seraphim","orcid":null},{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Kelly","contributor_surname":"Silva","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2011-09-01","storage_temperature":null,"cell_temperature":null,"exposure_time":null,"number_of_frames":null,"wavelength":null,"sample_detector_distance":null,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.162,"s_max":3.294,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBD3_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBD3_fit1_model1.pdb","model_mw":171.65,"bead_radius":5.0,"log":null,"symmetry":"","comment":"","user":161}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBD3_fit1_fit_img.png","software":null,"chi_square_value":1.892,"p_value":0.207525,"fit_residual_plot":"SASDBD3_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBD3_fit1.fit","fit_log":null,"software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from solutions of full length Leishmania braziliensis heat shock protein 90 (Hsp90) in 25 mM Tris 100 mM NaCl, 1 mM 2-mercaptoethanol were collected on the SAXS2 Beamline camera on the storage ring Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a MAR Image Plate detector (s = 4π sin θ/λ, where 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a single concentration scattering curve. ","tags":[],"intensity_unit":null,"experimental_mw":190.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.3825,"pddf_i0_error":0.113,"guinier_i0":0.37,"guinier_i0_error":null,"pddf_rg":5.56,"pddf_rg_error":0.023,"guinier_rg":5.32,"guinier_rg_error":null,"pddf_dmax":21.0,"pddf_dmax_error":null,"porod_volume":380.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":8,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBD3_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}