{"code":"SASDBF7","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions.","publication":{"title":"SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions.","author_list":"Santos SP, Cuypers MG, Round A, Finet S, Narayanan T, Mitchell EP, Romão CV","journal":"J Mol Biol","doi":"10.1016/j.jmb.2017.01.008","pmid":"28088481","published_date":"2017 Mar 10"},"status":"released","submitted_date":"2016-11-04","released_date":"2017-02-15"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBF7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBF7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBF7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBF7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBF7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBF7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBF7.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray 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Lisboa","webpage":"http://www.itqb.unl.pt"}],"contributor_name":"Sandra","contributor_surname":"Santos","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2013-11-22","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":1500,"wavelength":0.09919,"sample_detector_distance":2.81,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.063,"s_max":4.485,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBF7_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"2.1","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBF7_fit1_model1.pdb","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":"P23","comment":"","user":162}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBF7_fit1_fit_img.png","software":null,"chi_square_value":12.4,"p_value":0.0,"fit_residual_plot":"fit4columns620_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBF7_fit1.fit","fit_log":null,"software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBF7_fit2_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"2.2i","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBF7_fit2_model1.pdb","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":"P23","comment":"","user":162}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBF7_fit2_fit_img.png","software":null,"chi_square_value":3.02,"p_value":0.0,"fit_residual_plot":"SASDBF7_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBF7_fit2.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected from a sample of Dps2, (DNA binding protein under starvation conditions) using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) at the BM29 beam line on the ESRF storage ring (Grenoble, France). Data (1500 successive 1 second frames), were collected using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.09919 nm. The SEC mobile phase consisted of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5, (20°C) with a sample injection concentration of 10 mg/ml. Data obtained from solute-free eluates and SEC-elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the SEC-peak frames to produce the data displayed in this entry.","tags":[],"intensity_unit":null,"experimental_mw":261.71,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":261.71,"porod_mw_error":null,"pddf_i0":121.9,"pddf_i0_error":0.1,"guinier_i0":122.73,"guinier_i0_error":0.094,"pddf_rg":4.17,"pddf_rg_error":0.03,"guinier_rg":4.23,"guinier_rg_error":0.01,"pddf_dmax":12.74,"pddf_dmax_error":null,"porod_volume":445.08,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":10,"guinier_point_last":58,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBF7_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}