{"code":"SASDBH7","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions.","publication":{"title":"SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions.","author_list":"Santos SP, Cuypers MG, Round A, Finet S, Narayanan T, Mitchell EP, Romão CV","journal":"J Mol Biol","doi":"10.1016/j.jmb.2017.01.008","pmid":"28088481","published_date":"2017 Mar 10"},"status":"released","submitted_date":"2016-11-04","released_date":"2017-02-15"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBH7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBH7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBH7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBH7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBH7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBH7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBH7.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"N-terminal truncated DNA protection during starvation protein 1","short_name":"Dps1t","sequence":"HYLEEKEFQTVAETLQRNLATTISLYLKFKKYHWDIRGRFFRDLHLAYDEFIAEIFPSIDEQAERLVALG\r\nGSPLAAPADLARYSTVQVPQETVRDARTQVADLVQDLSRVGKGYRDDSQACDEANDPVTA\r\nDMYNGYAATIDKIRWMLQAIMDDERLD","organism":"Deinococcus radiodurans R1","uniprot_code":"Q9RS64","uniprot_range_first":51,"uniprot_range_last":207,"oligomerization":"dodecamer","molecular_type":"protein","uniprot_sequence":"MTKKSTKSEAASKTKKSGVPETGAQGVRAGGADHADAAHLGTVNNALVNHHYLEEKEFQT\nVAETLQRNLATTISLYLKFKKYHWDIRGRFFRDLHLAYDEFIAEIFPSIDEQAERLVALG\nGSPLAAPADLARYSTVQVPQETVRDARTQVADLVQDLSRVGKGYRDDSQACDEANDPVTA\nDMYNGYAATIDKIRWMLQAIMDDERLD","mw":18.037,"total_mw":216.444,"number_molecules":12,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris-HCl, 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Dps1 truncated, DNA binding protein under starvation conditions (SEC-SAXS)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Oeiras, Portugal","full_name":"Instituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa","webpage":"http://www.itqb.unl.pt"}],"contributor_name":"Sandra","contributor_surname":"Santos","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2013-11-22","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":1500,"wavelength":0.09919,"sample_detector_distance":2.81,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.063,"s_max":4.485,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBH7_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"2.2i","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBH7_fit1_model1.pdb","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":"P23","comment":"","user":162}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBH7_fit1_fit_img.png","software":null,"chi_square_value":10.6,"p_value":0.0,"fit_residual_plot":"SASDBH7_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBH7_fit1.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected from a sample of truncated Dps1, (DNA binding protein under starvation conditions) using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) at the BM29 beam line on the ESRF storage ring (Grenoble, France). Data (1500 successive 1 second frames), were collected using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.09919 nm. The SEC mobile phase consisted of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5, (20°C) with a sample injection concentration of 10 mg/ml. Data obtained from solute-free eluates and SEC-elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the SEC-peak frames to produce the data displayed in this entry.","tags":[],"intensity_unit":null,"experimental_mw":171.15,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":171.16,"porod_mw_error":null,"pddf_i0":52.14,"pddf_i0_error":0.04,"guinier_i0":52.35,"guinier_i0_error":0.048,"pddf_rg":3.83,"pddf_rg_error":0.003,"guinier_rg":3.88,"guinier_rg_error":0.01,"pddf_dmax":9.99,"pddf_dmax_error":null,"porod_volume":290.96,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":60,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBH7_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}