{"code":"SASDBJ2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.","publication":{"title":"Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.","author_list":"Li L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV","journal":"Mol Microbiol","doi":"10.1111/mmi.13735","pmid":"28628237","published_date":"2017 Sep"},"status":"released","submitted_date":"2015-10-01","released_date":"2017-05-23"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBJ2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBJ2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBJ2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBJ2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBJ2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBJ2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBJ2.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"40bp long dsDNA-Sa Oligonucleotide","short_name":"dsDNA-Sa Oligo","sequence":"GTAGTAAGATTTAAAAAAACATACTAAGATATAAATTAAC\r\n","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"DNA","uniprot_sequence":null,"mw":24.7,"total_mw":24.7,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family).","short_name":"ABfR1-WT","sequence":"MSIEITEKYVILKRFLSVAYGLSESEIDTFLRILESKEGKDIDAISAELKISKSRTSIIL KKLSDAGLVDKEKGDNMKGGRPKYVYSVNKEELKNRLIAKSEELCKELNQVIKSFI\r\n\r\n","organism":"Sulfolobus acidocaldarius","uniprot_code":"Q4JBH1","uniprot_range_first":1,"uniprot_range_last":116,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSIEITEKYVILKRFLSVAYGLSESEIDTFLRILESKEGKDIDAISAELKISKSRTSIIL\nKKLSDAGLVDKEKGDNMKGGRPKYVYSVNKEELKNRLIAKSEELCKELNQVIKSFI","mw":13.16,"total_mw":26.32,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"0.5 x Tris/Borate/EDTA (TBE)","concentration_unit":"v/v","comment":null,"additive":null,"concentration":0.5,"pka":null,"ph":null,"deuteration":null},"purity_method":null,"name":"Wild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDNA-Sa oligonucleotide.","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Marburg, Germany","full_name":"Philipps-Universität Marburg","webpage":""}],"contributor_name":"Ankan","contributor_surname":"Banerjee","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2015-08-27","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":20.0,"number_of_frames":10,"wavelength":0.93,"sample_detector_distance":2.43,"concentration_min":null,"concentration_max":3.5,"sample_volume":null,"flow_rate":null,"s_min":0.033,"s_max":4.934,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBJ2_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBJ2_fit1_model1.pdb","model_mw":null,"bead_radius":4.2,"log":null,"symmetry":"","comment":"","user":72}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBJ2_fit1_fit_img.png","software":null,"chi_square_value":1.55,"p_value":2e-06,"fit_residual_plot":"SASDBJ2_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBJ2_fit1.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from solutions of N terminal affinity tag cleaved (by thrombin) wild type AbfR1 in complex with SaOligo in 0.5 x TBE buffer was collected on the BM29 beam line on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). A single concentration of 3.5mg/ mL was measured in 10 successive 20 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted .The analysis was performed using the ATSAS package. The displayed model is the best fit model with the lowest reduced Chi-squared value (1.55) after imposing P2 symmetry. The DNA is considerably bent in complex with ABfR1.","tags":[],"intensity_unit":null,"experimental_mw":51.3,"experimental_mw_error":null,"guinier_i0_mw":47.93,"guinier_i0_mw_error":null,"porod_mw":41.0,"porod_mw_error":null,"pddf_i0":51.3,"pddf_i0_error":null,"guinier_i0":47.93,"guinier_i0_error":null,"pddf_rg":3.35,"pddf_rg_error":null,"guinier_rg":3.0,"guinier_rg_error":null,"pddf_dmax":11.76,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":52,"guinier_point_last":83,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBJ2_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}