{"code":"SASDBK6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"The C-terminal region of the human p23 chaperone modulates its structure and function.","publication":{"title":"The C-terminal region of the human p23 chaperone modulates its structure and function.","author_list":"Seraphim TV, Gava LM, Mokry DZ, Cagliari TC, Barbosa LR, Ramos CH, Borges JC","journal":"Arch Biochem Biophys","doi":"10.1016/j.abb.2014.10.015","pmid":"25447839","published_date":"2015 Jan 1"},"status":"released","submitted_date":"2016-09-13","released_date":"2016-10-05"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBK6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBK6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBK6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBK6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBK6_gxmzoOr_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBK6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBK6.sascif","experiment":{"instrument":{"detector":{"type":"20Hz","name":"Pilatus 300K","resolution":null},"name":"Brazilian Synchrotron Light Laboratory","city":"Campinas","country":"Brazil","beamline_name":"SAXS1 Beamline","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Prostaglandin E synthase 3 (1-131)","short_name":"PTGES, Sba1, p23","sequence":"GSMQPASAKWYDRRDYVFIEFCVEDSKDVNVNFEKSKLTFSCLGGSDNFKHLNEIDLFHCIDPNDSKHKRTDRSILCCLRK\r\nGESGQSWPRLTKERAKLNWLSVDFNNWKDWEDDSDEDMSNFDRFSEMMNNMG","organism":"Homo sapiens","uniprot_code":"Q15185","uniprot_range_first":1,"uniprot_range_last":131,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MQPASAKWYDRRDYVFIEFCVEDSKDVNVNFEKSKLTFSCLGGSDNFKHLNEIDLFHCID\nPNDSKHKRTDRSILCCLRKGESGQSWPRLTKERAKLNWLSVDFNNWKDWEDDSDEDMSNF\nDRFSEMMNNMGGDEDVDLPEVDGADDDSQDSDDEKMPDLE","mw":15.693,"total_mw":15.693,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Truncated construct of human p23 (1-131)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Júlio","contributor_surname":"Borges","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2013-06-21","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":30.0,"number_of_frames":2,"wavelength":0.1488,"sample_detector_distance":1.0,"concentration_min":1.0,"concentration_max":2.0,"sample_volume":null,"flow_rate":null,"s_min":0.108,"s_max":3.685,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"GNOM v4.5a","i0_calibration_standard":null,"description":"Samples were measured at 1 mg/mL and 2 mg/mL in 1 mm path-length mica cells.\r\nAll curves were inspected for X-ray damage and aggregation.\r\nThe experimental molecular weight was determined by analytical ultracentrifugation.","experiment_description":"Synchrotron SAXS data from solutions of truncated human p23 (1-131) in 25 mM Tris-HCl, 100 mM NaCl, 5 mM β-mercaptoethanol, pH 7.5 were collected on the SAXS1 Beamline at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a 20Hz Pilatus 300K detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.1488 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 2 mg/ml were measured at 20°C. Two successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":null,"experimental_mw":18.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.01632,"pddf_i0_error":2e-05,"guinier_i0":0.0163,"guinier_i0_error":3e-05,"pddf_rg":1.933,"pddf_rg_error":0.005,"guinier_rg":1.91,"guinier_rg_error":0.06,"pddf_dmax":7.0,"pddf_dmax_error":null,"porod_volume":31.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":71,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBK6_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}