{"code":"SASDBL4","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system.","publication":{"title":"Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system.","author_list":"Gleditzsch D, Müller-Esparza H, Pausch P, Sharma K, Dwarakanath S, Urlaub H, Bange G, Randau L","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkw469","pmid":"27216815","published_date":"2016 Jul 8"},"status":"released","submitted_date":"2016-03-18","released_date":"2016-09-01"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBL4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBL4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBL4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBL4.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Cas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family)","short_name":"Cas6f","sequence":"MNSYIDIRLKPDAEMREAELSSKVFTKFHKALVTLNSHKIGISFPQMKLSLGQLFRIHGDASLLHDLQGLDWLGPLAGYCQVTAVSAVPDHVQYRIVSVKRSNLSKAKLKRLIARGSIDKDGEKRYKVKMLGQGFDNPYLDLFSSSTGQVYRKFFEFSDIQAHPLDGEFDSYGLSKTATVPWF","organism":"Shewanella putrefaciens","uniprot_code":"A4Y6G3","uniprot_range_first":1,"uniprot_range_last":183,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MNSYIDIRLKPDAEMREAELSSKVFTKFHKALVTLNSHKIGISFPQMKLSLGQLFRIHGD\nASLLHDLQGLDWLGPLAGYCQVTAVSAVPDHVQYRIVSVKRSNLSKAKLKRLIARGSIDK\nDGEKRYKVKMLGQGFDNPYLDLFSSSTGQVYRKFFEFSDIQAHPLDGEFDSYGLSKTATV\nPWF","mw":20.674,"total_mw":20.674,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822)","short_name":"Cas5fv","sequence":"MKIIIEYDSCWRNAFLGGSNNEPVPKKGREFLGSMTSLKKEGNFKVCENTLDTVMGVLNRLIGDQRKLYQARSKMYESAYYFEALEDKVSFIDKPQLTNEISFIRNMNGSTDQNAFTGMIKVSDPVFTSEYSQQFWGVLALDFTQLCDFIIKQSQVVGSIELNPLSIINRLESLNQEKALENSDDLAQVLKVLNEYFPDIEYLNNKGLITPISIYCSALYLQLARLETSFNMTTAKTKAGGISGISKRGFTKKDFMDRYTTGPKKTIWGNPFIKKEKIKGQGEVTSMMTKASGQLEISIDVDRDKAQEIKILIENAGVSSFYLGKKGLAYVSNIKL","organism":"Shewanella putrefaciens","uniprot_code":"A4Y6G2","uniprot_range_first":1,"uniprot_range_last":336,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MKIIIEYDSCWRNAFLGGSNNEPVPKKGREFLGSMTSLKKEGNFKVCENTLDTVMGVLNR\nLIGDQRKLYQARSKMYESAYYFEALEDKVSFIDKPQLTNEISFIRNMNGSTDQNAFTGMI\nKVSDPVFTSEYSQQFWGVLALDFTQLCDFIIKQSQVVGSIELNPLSIINRLESLNQEKAL\nENSDDLAQVLKVLNEYFPDIEYLNNKGLITPISIYCSALYLQLARLETSFNMTTAKTKAG\nGISGISKRGFTKKDFMDRYTTGPKKTIWGNPFIKKEKIKGQGEVTSMMTKASGQLEISID\nVDRDKAQEIKILIENAGVSSFYLGKKGLAYVSNIKL","mw":37.861,"total_mw":37.861,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Hexameric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821)","short_name":"Cas7fv-hexamer","sequence":"MGSSHHHHHHSQNPMQKVTGIKSVDFKIKALGHGVVNWNGPTTLTGDDGKTVDNHTLPKLRGYTNLTGKVKDETGYKYKKQATDINFKETPLYISQNCIRHHLFREQAFDLHYASDKNLKNVLASITGLIRGYVVPSSQCKRTSPLLLEDFVDQLGNGNFEQYGQAGARDSTSFFSKTTFGDTEYISYGSISIEQLQFISLDKKFDRAAMVIKEGEGEVIAAELQNYIQSLNPSLNPQAIFHSNYVRRGTIFEEGECGILLNDDAVKALVAETLERLANLSIRQAKGYMYVDDITVDYNDSHKMMRIKRDESEIINEQHAPFAQYFYAK\r\n","organism":"Shewanella putrefaciens","uniprot_code":"A4Y6G1","uniprot_range_first":1,"uniprot_range_last":315,"oligomerization":"hexamer","molecular_type":"protein","uniprot_sequence":"MQKVTGIKSVDFKIKALGHGVVNWNGPTTLTGDDGKTVDNHTLPKLRGYTNLTGKVKDET\nGYKYKKQATDINFKETPLYISQNCIRHHLFREQAFDLHYASDKNLKNVLASITGLIRGYV\nVPSSQCKRTSPLLLEDFVDQLGNGNFEQYGQAGARDSTSFFSKTTFGDTEYISYGSISIE\nQLQFISLDKKFDRAAMVIKEGEGEVIAAELQNYIQSLNPSLNPQAIFHSNYVRRGTIFEE\nGECGILLNDDAVKALVAETLERLANLSIRQAKGYMYVDDITVDYNDSHKMMRIKRDESEI\nINEQHAPFAQYFYAK","mw":37.168,"total_mw":223.01,"number_molecules":6,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"wildtype-crRNA: CRISPR/Cas Type I-F Cascade component","short_name":"wildtype-crRNA","sequence":"CUUAGAAAUAUCGCCCAGCAAGACGCGCAAACCUAUAACCGUUCACCGCCGCACAGGCGG","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"RNA","uniprot_sequence":null,"mw":19.29,"total_mw":19.29,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM HEPES 150 mM NaCl 1mM DTT 1mM EDTA","concentration_unit":"mM","comment":null,"additive":"150 mM NaCl, 1mM DTT, 1mM EDTA","concentration":50.0,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"The 1:1:6:1 crRNA:Cas6f:Cas7fv:Cas5fv CRISPR/Cas Type I-F wild-type Cascade complex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Marburg, Germany","full_name":"University of Marburg","webpage":"http://www.uni-marburg.de/"}],"contributor_name":"Patrick","contributor_surname":"Pausch","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2015-06-27","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":1.0,"number_of_frames":10,"wavelength":0.93,"sample_detector_distance":2.43,"concentration_min":null,"concentration_max":20.0,"sample_volume":null,"flow_rate":null,"s_min":0.033,"s_max":4.934,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBL4_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBL4_fit1_model1.pdb","model_mw":null,"bead_radius":3.8,"log":null,"symmetry":"","comment":"","user":118}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBL4_fit1_fit_img.png","software":null,"chi_square_value":2.37,"p_value":0.0003,"fit_residual_plot":"SASDBL4_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBL4_fit1.fir","fit_log":null,"software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBL4_fit2_model1_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBL4_fit2_model1.pdb","model_mw":null,"bead_radius":4.75,"log":null,"symmetry":"","comment":"","user":118}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBL4_fit2_fit_img.png","software":null,"chi_square_value":2.18,"p_value":0.0003,"fit_residual_plot":"SASDBL4_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBL4_fit2.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the 1:1:6:1 crRNA:Cas6f:Cas7fv:Cas5fv CRISPR/Cas Type I-F Cascade complex in 50 mM HEPES 150 mM NaCl, 1mM DTT, 1mM EDTA, pH 7 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 2M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.124 nm). The data displayed in this entry were measured from one sample at a solute concentration of 20 mg/ml using an exposure time of 10 s (collected as 10 x 1 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were normalized to protein concentration. ","tags":[],"intensity_unit":null,"experimental_mw":225.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":160.4,"pddf_i0_error":0.06,"guinier_i0":160.6,"guinier_i0_error":0.1,"pddf_rg":5.44,"pddf_rg_error":0.03,"guinier_rg":5.43,"guinier_rg_error":0.06,"pddf_dmax":18.4,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":11,"guinier_point_last":34,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBL4_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}