{"code":"SASDBL7","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Vaccinia Virus Immunomodulator A46: A Lipid and Protein-Binding Scaffold for Sequestering Host TIR-Domain Proteins.","publication":{"title":"Vaccinia Virus Immunomodulator A46: A Lipid and Protein-Binding Scaffold for Sequestering Host TIR-Domain Proteins.","author_list":"Fedosyuk S, Bezerra GA, Radakovics K, Smith TK, Sammito M, Bobik N, Round A, Ten Eyck LF, Djinović-Carugo K, Usón I, Skern T","journal":"PLoS Pathog","doi":"10.1371/journal.ppat.1006079","pmid":"27973613","published_date":"2016 Dec"},"status":"released","submitted_date":"2016-11-06","released_date":"2016-11-23"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBL7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBL7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBL7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBL7.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Protein A46","short_name":"A46-delta","sequence":"GSMAQQMAFDISVNASKTINALVYFSTQQNKLVIRNEVNDTHYTVEFDRDKVVDTFISYNRHNDTIEIRGVLPEET\r\nNIGCAVNTPVSMT","organism":"Vaccinia virus","uniprot_code":"P26672","uniprot_range_first":1,"uniprot_range_last":83,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MAFDISVNASKTINALVYFSTQQNKLVIRNEVNDTHYTVEFDRDKVVDTFISYNRHNDTI\nEIRGVLPEETNIGCAVNTPVSMTYLYNKYSFKLILAEYIRHRNTISGNIYSALMTLDDLA\nIKQYGDIDLLFNEKLKVDSDSGLFDFVNFVKDMICCDSRIVVALSSLVSKHWELTNKKYR\nCMALAEHISDSIPISELSRLRYNLCKYLRGHTESIEDKFDYFEDDDSSTCSAVTDRETDV","mw":10.028,"total_mw":40.112,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris-HCl, 10 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.5,"deuteration":null},"purity_method":null,"name":"N-terminal domain of Vaccinia virus A46 protein (1-83)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Vienna, Austria","full_name":"Medical University of Vienna","webpage":"https://www.meduniwien.ac.at"}],"contributor_name":"Sofiya","contributor_surname":"Fedosyuk","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-06-25","storage_temperature":null,"cell_temperature":20.0,"exposure_time":0.1,"number_of_frames":null,"wavelength":0.09918,"sample_detector_distance":2.867,"concentration_min":null,"concentration_max":15.5,"sample_volume":null,"flow_rate":null,"s_min":0.131,"s_max":4.934,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBL7_fit1_model1_img.png","software":"CORAL","pdb_link":[{"pdb_code":"5ezu","ext_code":"5EZU","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"mix","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBL7_fit1_model1.pdb","model_mw":42.86,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/tetramerFull00.log","symmetry":"","comment":"","user":176}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBL7_fit1_fit_img.png","software":null,"chi_square_value":0.657,"p_value":0.0,"fit_residual_plot":"SASDBL7_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBL7_fit1.fit","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"GNOM v5","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected from a sample of the N-terminal domain of Vaccinia virus A46 protein (1-83) using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS; Superdex 200 10/300 column) at the BM29 beam line on the ESRF storage ring (Grenoble, France). Data were collected using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09918 nm. The SEC mobile phase consisted of 20 mM Tris-HCl, 10 mM DTT, pH 8.5, (20°C) with a sample injection concentration of 15.5 mg/ml. Data obtained from solute-free eluates and SEC-elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the SEC-peak frames to produce the data displayed in this entry.","tags":[],"intensity_unit":null,"experimental_mw":38.857,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":38.857,"porod_mw_error":null,"pddf_i0":14.97,"pddf_i0_error":0.03,"guinier_i0":14.91,"guinier_i0_error":0.02,"pddf_rg":2.65,"pddf_rg_error":0.01,"guinier_rg":2.61,"guinier_rg_error":0.09,"pddf_dmax":9.0,"pddf_dmax_error":null,"porod_volume":68.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":65,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBL7_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}