{"code":"SASDBN6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"TIA-1 RRM23 binding and recognition of target oligonucleotides.","publication":{"title":"TIA-1 RRM23 binding and recognition of target oligonucleotides.","author_list":"Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkx102","pmid":"28184449","published_date":"2017 May 5"},"status":"released","submitted_date":"2016-09-22","released_date":"2017-03-28"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBN6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBN6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBN6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBN6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBN6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBN6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBN6.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Australian  Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":"Point","type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Nucleolysin TIA-1 isoform p40","short_name":"TIA-1","sequence":"ENLYFQGAMGNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGK","organism":"Homo sapiens","uniprot_code":"P31483","uniprot_range_first":93,"uniprot_range_last":274,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAA\nAALAAMNGRKIMGKEVKVNWATTPSSQKKDTSSSTVVSTQRSQDHFHVFVGDLSPEITTE\nDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTN\nWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQI\nMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDMINPVQQQNQI\nGYPQPYGQWGQWYGNAQQIGQYMPNGWQVPAYGMYGQAWNQQGFNQTQSSAPWMGPNYGV\nQPPQGQNGSMLPNQPSGYRVAGYETQ","mw":21.325,"total_mw":21.325,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"RNA (ACUCCUUUUU)","short_name":null,"sequence":"ACUCCUUUUU","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"RNA","uniprot_sequence":null,"mw":0.999,"total_mw":0.999,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES, 100 mM NaCl, 3% v/v glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Nucleolysin TIA-1 isoform p40 in complex with ACUCCUUUUU RNA","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Melbourne, Australia","full_name":"Monash University","webpage":"http://www.monash.edu/"}],"contributor_name":"Matthew","contributor_surname":"Wilce","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-05-27","storage_temperature":15.0,"cell_temperature":15.0,"exposure_time":1.0,"number_of_frames":30,"wavelength":0.1033,"sample_detector_distance":1.6,"concentration_min":null,"concentration_max":2.5,"sample_volume":null,"flow_rate":null,"s_min":0.224,"s_max":3.004,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"GNOM 4.6","i0_calibration_standard":null,"description":"TIA-1 RRM23 + CU-rich RNA","experiment_description":"Synchrotron SAXS data from solutions of Nucleolysin TIA-1 isoform p40 in complex with RNA (ACUCCUUUUU) in 20 mM HEPES, 100mM NaCl, 3% v/v glycerol, pH 7 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The data were collected as 30 successive 1 second frames and were normalized to the intensity of the transmitted beam and radially averaged. The radially-averaged scattering of the solvent-blank was subtracted to produce the results displayed in this entry that shows the scattering profile from a sample at 2.5 mg/ml maintained at 15°C during the SAXS measurements.","tags":[],"intensity_unit":null,"experimental_mw":23.7,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.0556,"pddf_i0_error":null,"guinier_i0":0.056,"guinier_i0_error":null,"pddf_rg":2.15,"pddf_rg_error":null,"guinier_rg":2.17,"guinier_rg_error":null,"pddf_dmax":6.6,"pddf_dmax_error":null,"porod_volume":29.8,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":82,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBN6_datloglog_img.png","symmetry":null,"last_modified":"2020-07-22T10:39:57.780614+02:00","bragg_peak":[]}