{"code":"SASDBN9","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"YeeF, a polymorphic toxin from Bacillus subtilis, is a ribosomal RNase with promiscuous DNA binding property","publication":null,"status":"released","submitted_date":"2017-01-05","released_date":"2021-09-20"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBN9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBN9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBN9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBN9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBN9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBN9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBN9.sascif","experiment":{"instrument":{"detector":{"type":"1D diode array detector","name":"Mythen 1K","resolution":50.0},"name":"CSIR - Institute of Microbial Technology (IMTech)","city":"Chandigarh","country":"India","beamline_name":"Anton Paar SAXSpace","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Putative DNA binding protein","short_name":"YeeF","sequence":"M G S S H H H H H H S S G L V P R S H M A S IYKTKEGYTYTTDNYGRITSVKADLQLGEAKRN\r\nQYAQSHAGKPQDRKPDDDGGALIATQFKGSGQFDNIVPMNSQINRSGGRWYEMEQEWAKALKEEPPQKVN\r\nVNIKAIYKGDSLRPDKFIVKFRIGDADFEKVTIKNQSGG","organism":"Bacillus subtilis subsp. spizizenii","uniprot_code":"E0TU96","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MKVLEVKTLLSEATDRAKEYKELRTQMVNLRKALKSVADLGDSEFSGKGASNIKAFYHDH\nVGVTDQWIDYIDMKIAFFNSIAGAAEDKGLSDAYIEESFLEHELANAHKKSKSIMSEQKK\nAMKDILNDIDNILPLDLFSTETFNDELADANDKRKKTLEKLDALDEDLKTEYALSEPNEQ\nFIKSDFQKLQEATGKGKNATPIHYNAKAYRESDIHKKKGDIEKRTEAYLKIKKEEAKERE\nIEKLKERLKNYDYADADEFYEMAKTIGYENLTAEQQRYFTQIENTRELEAGFKGVAVGLY\nDSGKDAVVGLWDMVTDPGGTVEAITGAVAHPIKTYEAISAAIEESYQKDMVNGDTYSRAR\nWVSYAVGTVVTSIVGTKGVGAVSKTGTATKVTTKVKTAASKSATAQKAITVSKQTIDHIK\nQKVNKGIEVSKKHVKTKLNQIGDLTLADILPYHPRHDLVPAGVPYNAVNGVTLKEGLQKF\nAKVILPKPYGTSSSGRRTPAPVVPPVTVKYGEHYARWSRKKVLKPNIIYKTKEGYTYTTD\nNYGRITSVKADLQLGEAKRNQYAQSHAGKPQDRKPDDDGGHLIATQFKGSGQFDNIVPMN\nSQINRSGGRWYEMEQEWAKALKEEPPQKVNVNIKAIYKGDSLRPDKFIVKFRIGDADFEK\nVTIKNQSGG","mw":18.402,"total_mw":36.804,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"YezG, cognate  immunity protein  of YeeF","short_name":"YezG","sequence":"MDTPKMGDLYQRIANQINEMIPSEWENVYLYAEILDDSSEVYFYFNIPGKNEFLYSHNIPEHFNVSEDIY\r\nDDLLIELQESFEELREEYEKNNPETWTNLTLKLDRTGQFSIDYNYEDVIASELNGSQRKAVWVYKNLGLM\r\nPKRKTVRDFLEDYIKTNEGKI","organism":"Bacillus subtilis subsp. spizizenii","uniprot_code":"E0TU95","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MDTPKMGDLYQRIANQINEMIPSEWENVYLYAEILDDSSEVYFYFNIPGKNEFLYSHNIP\nEHFNVSEDIYDDLLIELQESFEELREEYEKNNPETWTNLTLKLDRTGQFSIDYNYEDVIA\nSELNGSQRKAVWVYKNLGLMPKRKTVRDFLEDYIKTNEGKI","mw":19.234,"total_mw":19.234,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Putative DNA binding protein YeeF-CT in complex with the cognate immunity protein YezG from Bacillus subtilis","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"CSIR","address":"Chandigarh, India","full_name":"CSIR-Institute of Microbial Technology","webpage":"http://www.imtech.res.in/"}],"contributor_name":"Krishan","contributor_surname":"Gopal","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-08-18","storage_temperature":4.0,"cell_temperature":25.0,"exposure_time":1800.0,"number_of_frames":1,"wavelength":0.15418,"sample_detector_distance":null,"concentration_min":null,"concentration_max":7.5,"sample_volume":null,"flow_rate":null,"s_min":0.121,"s_max":7.001,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBN9_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"2.7.2","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBN9_fit1_model1.pdb","model_mw":80.816,"bead_radius":4.0,"log":"https://www.sasbdb.org/media/log_files/YeeF-CT_YezG.log","symmetry":"","comment":"","user":196}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBN9_fit1_fixed_fit_img.png","software":"DAMMIN","chi_square_value":0.812,"p_value":0.0,"fit_residual_plot":"SASDBN9_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBN9_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/YeeF-CT_YezG.log","software_version":"2.7.2","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"ATSAS 2.7.2","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of Putative DNA binding protein YeeF-CT complexed with the cognate immunity protein YezG from Bacillus subtilis in 20 mM HEPES 150 mM NaCl, pH 7.5 were collected on the IMTECH camera (Chandigarh, India) using a Diode 1D CMOS Mythen detector at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). A single solute concentration of 7.5 mg/ml was measured at 25°C for 1800 seconds. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to produce the final scattering profile for this entry.","tags":[],"intensity_unit":null,"experimental_mw":72.03,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":72.03,"porod_mw_error":null,"pddf_i0":72150.0,"pddf_i0_error":1510.0,"guinier_i0":72749.0,"guinier_i0_error":2036.0,"pddf_rg":4.15,"pddf_rg_error":0.09,"guinier_rg":4.04,"guinier_rg_error":0.18,"pddf_dmax":14.1,"pddf_dmax_error":null,"porod_volume":115.26,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":2,"guinier_point_last":32,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBN9_datloglog_img.png","symmetry":null,"last_modified":"2021-09-20T13:31:35.716414+02:00","bragg_peak":[]}