{"code":"SASDBP8","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.","publication":{"title":"Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.","author_list":"Shah N, Beckham SA, Wilce JA, Wilce MCJ","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkx1307","pmid":"29346611","published_date":"2018 Apr 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GNOM","pddf_software_version":"GNOM v4.5","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Probable ATP-dependent RNA helicase DDX58 (Delta-CARDs) plus 8mer hairpin dsRNA/ADP-AlFx (SEC-peak1) in 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH 7.4 were collected using size exclusion chromatography (SEC-SAXS) on the SAXS/WAXS camera on the storage ring Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.10332 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Data frames (17 x 2 s exposures) were collected through the SEC elution peak at 36 min (GE Healthcare Life Sciences Superdex 200 10/300; 0.4 ml/min; 100 μl injection at 8 mg/ml). The data were normalized to the intensity of the transmitted beam and radially averaged; the radially averaged scattering of the solvent-blank was subtracted and the different curves were scaled to yield the final composite scattering curve. The SAXS data were collected at 15 °C.","tags":[],"intensity_unit":null,"experimental_mw":86.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":77.6,"porod_mw_error":null,"pddf_i0":0.0219,"pddf_i0_error":null,"guinier_i0":0.0219,"guinier_i0_error":null,"pddf_rg":2.98,"pddf_rg_error":null,"guinier_rg":2.96,"guinier_rg_error":null,"pddf_dmax":10.5,"pddf_dmax_error":null,"porod_volume":131.92,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":18,"guinier_point_last":92,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBP8_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}