{"code":"SASDBQ4","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Structural basis of the interaction between the putative adhesion-involved and iron-regulated FrpD and FrpC proteins of Neisseria meningitidis.","publication":{"title":"Structural basis of the interaction between the putative adhesion-involved and iron-regulated FrpD and FrpC proteins of Neisseria meningitidis.","author_list":"Sviridova E, Rezacova P, Bondar A, Veverka V, Novak P, Schenk G, Svergun DI, Kuta Smatanova I, Bumba L","journal":"Sci Rep","doi":"10.1038/srep40408","pmid":"28084396","published_date":"2017 Jan 13"},"status":"released","submitted_date":"2016-04-05","released_date":"2017-01-26"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBQ4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBQ4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBQ4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBQ4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBQ4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBQ4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBQ4.sascif","experiment":{"instrument":{"detector":{"type":"Custom-built","name":"Pilatus 1M-W","resolution":0.172},"name":"DORIS III, DESY","city":"Hamburg","country":"Germany","beamline_name":"EMBL X33","beam_geometry":"0.60","type_of_source":"X-ray 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mM Tris-HCl 150 mM NaCl 0.01% NaN3","concentration_unit":"mM","comment":null,"additive":"150 mM NaCl, 0.01% NaN3","concentration":50.0,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Neisseria meningitidis iron-regulated FrpD-FrpC lipoprotein/protein complex ","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Stanford, California, USA","full_name":"Stanford University","webpage":"https://www.stanford.edu/"}],"contributor_name":"Gundolf","contributor_surname":"Schenk","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2011-10-19","storage_temperature":10.0,"cell_temperature":null,"exposure_time":15.0,"number_of_frames":8,"wavelength":0.15,"sample_detector_distance":2.7,"concentration_min":0.75,"concentration_max":4.9,"sample_volume":null,"flow_rate":null,"s_min":0.085,"s_max":6.032,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBQ4_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBQ4_fit1_model1.pdb","model_mw":82.0,"bead_radius":3.0,"log":null,"symmetry":"","comment":"","user":122}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBQ4_fit1_fit_img.png","software":null,"chi_square_value":1.15,"p_value":0.029,"fit_residual_plot":"SASDBQ4_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBQ4_fit1.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from solutions of the 1:1 FrpD-FrpC complex from Neisseria meningitidis in 50 Tris-HCl, 150 mM NaCl, 0.01% NaN3 were collected on the X33 beam line at the DORIS storage ring (DESY, Hamburg, Germany) using a Pilatus 1M-W detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.15 nm). The data displayed in this entry shows the merged and extrapolated to zero-concentration SAXS profile derived from solute concentrations measured across the range of 0.75-4.90 mg/ml. The SAXS data for each sample were measured using an exposure time of 120 s (collected as 8 x 15 s frames) and were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted and the resulting subtracted data were scaled to each respective protein concentration prior to merging.","tags":["X33"],"intensity_unit":null,"experimental_mw":40.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":74.0,"porod_mw_error":null,"pddf_i0":50.7,"pddf_i0_error":0.15,"guinier_i0":50.3,"guinier_i0_error":0.4,"pddf_rg":3.86,"pddf_rg_error":0.01,"guinier_rg":3.72,"guinier_rg_error":0.4,"pddf_dmax":13.5,"pddf_dmax_error":null,"porod_volume":123.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":35,"guinier_point_last":97,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBQ4_datloglog_img.png","symmetry":null,"last_modified":"2023-05-25T11:21:52.654103+02:00","bragg_peak":[]}