{"code":"SASDBS6","status":"Published","type_of_curve":"Other","angular_unit":"1/A","project":{"title":"Structural and biochemical analysis of <i>Escherichia coli</i> ObgE, a central regulator of bacterial persistence.","publication":{"title":"Structural and biochemical analysis of <i>Escherichia coli</i> ObgE, a central regulator of bacterial persistence.","author_list":"Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W","journal":"J Biol Chem","doi":"10.1074/jbc.M116.761809","pmid":"28223358","published_date":"2017 Apr 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GNOM","pddf_software_version":"GNOM 5.0 (r4547)","i0_calibration_standard":null,"description":"SAXS-based ensemble modelling of ObgE_FL taking into account the flexibility of the C-terminal\r\ndomain.\r\nThe sample protein includes a C-terminal Strep-tag; frames corresponding to the peak in HPLC-SAXS run were averaged using DATAVER v0.2 (r3709)","experiment_description":"Synchrotron SAXS data from solutions of the Apo form of full length ObgE from E.coli (ObgE_FL) in 20 mM HEPES , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH 7.5, were collected at the SWING beam line at the SOLEIL Synchrotron (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Data collection was performed at at 10°C. 255 successive 2.8 second frames were collected through an HPLC-SEC elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration, then averaged to produce the final SAXS profile displayed in this entry.","tags":[],"intensity_unit":null,"experimental_mw":55.4,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":60.0,"porod_mw_error":null,"pddf_i0":0.03904,"pddf_i0_error":0.0003,"guinier_i0":0.0383,"guinier_i0_error":2.7e-05,"pddf_rg":3.96,"pddf_rg_error":0.1,"guinier_rg":3.7,"guinier_rg_error":0.1,"pddf_dmax":18.1,"pddf_dmax_error":null,"porod_volume":102.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":18,"guinier_point_last":46,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBS6_datloglog_img.png","symmetry":null,"last_modified":"2020-08-28T15:28:07.504745+02:00","bragg_peak":[]}