{"code":"SASDBS7","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core.","publication":{"title":"Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core.","author_list":"Chen Y, Tokuda JM, Topping T, Meisburger SP, Pabit SA, Gloss LM, Pollack L","journal":"Proc Natl Acad Sci U S A","doi":"10.1073/pnas.1611118114","pmid":"28028239","published_date":"2017 Jan 10"},"status":"released","submitted_date":"2016-10-27","released_date":"2017-02-15"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBS7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBS7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBS7_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBS7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBS7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"Advanced Photon Source (APS), Argonne National Laboratory","city":"Lemont, IL","country":"USA","beamline_name":"BioCAT 18ID","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer","short_name":null,"sequence":"GKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGRVTIAQGGVLPNIQSVLLPKK\r\n\r\nKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK","organism":"Xenopus laevis","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":null,"mw":23.754,"total_mw":47.508,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer","short_name":null,"sequence":"LATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA\r\n\r\nKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG","organism":"Xenopus laevis","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":null,"mw":23.168,"total_mw":46.336,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Nucleasome Core Particle with Widom 601 DNA - dsDNA","short_name":null,"sequence":"ATCGGGATGTATATATCTGACACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATTCTCCGAT\r\n \r\n\r\nATCGGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCCCGAT","organism":"Xenopus laevis","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"DNA","uniprot_sequence":null,"mw":92.093,"total_mw":92.093,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris-Cl, 0.1 mM EDTA, 0.1 mM DTT, 50% sucrose, 1.2 M NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Nucleosome Core Particle: 1.2 M NaCl, 50% sucrose, 50ms - 10s time resolved data","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Ithaca, NY, United States","full_name":"Cornell University","webpage":"https://www.cornell.edu/"}],"contributor_name":"Yujie","contributor_surname":"Chen","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2014-04-14","storage_temperature":0.0,"cell_temperature":20.0,"exposure_time":0.01,"number_of_frames":5,"wavelength":0.125,"sample_detector_distance":2.5,"concentration_min":null,"concentration_max":3.0,"sample_volume":null,"flow_rate":null,"s_min":0.06,"s_max":1.95,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":"From the depositor:\r\n\"Protein signal is blanked out in 50% sucrose, only DNA contributes to the scattering. Hence, the SAXS profile reports the Rg and molecule weight of the DNA in the nucleosome core particle complex.\"","experiment_description":"Synchrotron SAXS data from solutions of Nucleasome Core Particle in 20 mM Tris-HCl, 0.1 mM EDTA, 0.1 mM DTT, 50% sucrose, 1.2 M NaCl, pH 7.5, were collected on the 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 100K detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.125 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 20°C through a time-course spanning 50 ms to 10 s (with a 50 ms delay time). The data displayed in this entry represent the scattering after 50 ms; the remaining data from the time series can be located in the full-entry zip archive.","tags":[],"intensity_unit":null,"experimental_mw":184.0,"experimental_mw_error":null,"guinier_i0_mw":84.01,"guinier_i0_mw_error":1.7,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":307000.0,"guinier_i0_error":12400.0,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":5.81,"guinier_rg_error":0.38,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":11,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBS7_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}