{"code":"SASDBT2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.","publication":{"title":"Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.","author_list":"Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J","journal":"Structure","doi":"10.1016/j.str.2015.12.001","pmid":"26774128","published_date":"2016 Feb 2"},"status":"released","submitted_date":"2015-12-03","released_date":"2016-07-03"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBT2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBT2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBT2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBT2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBT2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBT2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBT2.sascif","experiment":{"instrument":{"detector":{"type":"CCD","name":"AVIEX PCCD170170","resolution":null},"name":"SOLEIL","city":"Saint-Aubin","country":"France","beamline_name":"SWING","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Ankyrin repeat domains from Tankyrase 2","short_name":"Tankyrase-2(489-649)","sequence":"GNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAAL","organism":"Homo sapiens","uniprot_code":"Q9H2K2","uniprot_range_first":489,"uniprot_range_last":649,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSGRRCAGGGAACASAAAEAVEPAARELFEACRNGDVERVKRLVTPEKVNSRDTAGRKST\nPLHFAAGFGRKDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNAR\nDNWNYTPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDGRTALDLADPSAKAVLTGEYKKDE\nLLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAK\nDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSYG\nADPTLLNCHNKSAIDLAPTPQLKERLAYEFKGHSLLQAAREADVTRIKKHLSLEMVNFKH\nPQTHETALHCAAASPYPKRKQICELLLRKGANINEKTKEFLTPLHVASEKAHNDVVEVVV\nKHEAKVNALDNLGQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQL\nLQEGISLGNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVS\nVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAA\nAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLAR\nVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAA\nSYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQT\nPLDLVSADDVSALLTAAMPPSALPSCYKPQVLNGVRSPGATADALSSGPSSPSSLSAASS\nLDNLSGSFSELSSVVSSSGTEGASSLEKKEVPGVDFSITQFVRNLGLEHLMDIFEREQIT\nLDVLVEMGHKELKEIGINAYGHRHKLIKGVERLISGQQGLNPYLTLNTSGSGTILIDLSP\nDDKEFQSVEEEMQSTVREHRDGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEEN\nHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTG\nCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYV\nIYRGEQAYPEYLITYQIMRPEGMVDG","mw":17.565,"total_mw":17.565,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM HEPES 100mM NaCl 1mM TCEP","concentration_unit":"mM","comment":null,"additive":"100mM NaCl, 1mM TCEP","concentration":50.0,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Ankyrin repeat domains from human Tankyrase-2 (489-649)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"Soleil","address":"Saint-Aubin, France","full_name":"Soleil Synchrotron","webpage":"http://www.synchrotron-soleil.fr/"}],"contributor_name":"Aurélien","contributor_surname":"Thureau","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2014-12-04","storage_temperature":15.0,"cell_temperature":null,"exposure_time":1.5,"number_of_frames":null,"wavelength":0.1,"sample_detector_distance":1.82,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.062,"s_max":6.083,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from solutions of the Ankyrin repeat domains from human Tankyrase-2 (amino acids 489-649) in 50mM HEPES, pH 7.5, 100mM NaCl, 1mM TCEP were collected at 15 C on the SWING Beamline, Synchrotron Soleil, Gif Sur Yvette, France using a PCCD170170 Aviex detector. These data are derived from size-exclusion chromatography-SAXS. Note: The original SAXS data where the momentum transfer s in inverse Angstroms (s = 4π sin θ/λ, where 2θ is the scattering angle) is included in the zip file associated with this entry.","tags":[],"intensity_unit":null,"experimental_mw":17.1,"experimental_mw_error":null,"guinier_i0_mw":17.1,"guinier_i0_mw_error":null,"porod_mw":15.0,"porod_mw_error":null,"pddf_i0":0.0444,"pddf_i0_error":2e-05,"guinier_i0":0.044,"guinier_i0_error":3e-05,"pddf_rg":1.84,"pddf_rg_error":0.001,"guinier_rg":1.77,"guinier_rg_error":0.01,"pddf_dmax":6.25,"pddf_dmax_error":null,"porod_volume":23.6,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":23,"guinier_point_last":111,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBT2_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}