{"code":"SASDBT4","status":"Published","type_of_curve":"Other","angular_unit":"1/nm","project":{"title":"Bovine Serum Albumin measured by SEC-SAXS","publication":null,"status":"released","submitted_date":"2016-04-11","released_date":"2016-04-14"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBT4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBT4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBT4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBT4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBT4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBT4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBT4.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Bovine serum albumin, monomer","short_name":"BSA monomer","sequence":"DTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFA\r\nKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEPERNE\r\nCFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYA\r\nPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREKVLTSSARQRLRCA\r\nSIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLL\r\nECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPEN\r\nLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAK\r\nEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEY\r\nGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTED\r\nYLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKA\r\nFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENF\r\nVAFVDKCCAADDKEACFAVEGPKLVVSTQTALA","organism":"Bos taurus","uniprot_code":"P02769","uniprot_range_first":25,"uniprot_range_last":607,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MKWVTFISLLLLFSSAYSRGVFRRDTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPF\nDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEP\nERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYY\nANKYNGVFQECCQAEDKGACLLPKIETMREKVLASSARQRLRCASIQKFGERALKAWSVA\nRLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKE\nCCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRR\nHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEK\nLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLIL\nNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLP\nDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVV\nSTQTALA","mw":66.462,"total_mw":66.462,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris 142 mM NaCl 5 % Glycerol 1mM DTT","concentration_unit":"mM","comment":null,"additive":"142 mM NaCl, 5 % Glycerol, 1mM DTT","concentration":20.0,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Bovine serum albumin monomer (measured by SEC-SAXS)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL","address":"Grenoble, France","full_name":"European Molecular Biology Laboratory (EMBL) - Grenoble Outstation","webpage":"https://www.embl.fr/"}],"contributor_name":"Martha","contributor_surname":"Brennich","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2016-02-22","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":40,"wavelength":0.93,"sample_detector_distance":2.43,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.132,"s_max":4.865,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBT4_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"3v03","ext_code":"3V03","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBT4_fit1_model1.pdb","model_mw":62.38,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":119}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBT4_fit1_fixed_fit_img.png","software":null,"chi_square_value":2.41,"p_value":0.0,"fit_residual_plot":"SASDBT4_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBT4_fit1.fit","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of monomeric bovine serum albumin in 20 mM Tris, 142 mM NaCl, 5 % Glycerol, 1mM DTT, pH 7 were collected on the BM29 SAXS beam line at the ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector  (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.099 nm). Forty successive 1 s frames were collected through a BSA elution peak from a size-exclusion chromatography column (GE Healthcare Superdex 200 10/300 GL at 0.5 ml/min; initial load concentration = 10 mg/ml). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank (derived from the SEC running solvent) was subtracted and the individual solvent-corrected SAXS profiles were scaled and averaged.","tags":["benchmark"],"intensity_unit":null,"experimental_mw":58.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":69.0,"porod_mw_error":null,"pddf_i0":152.1,"pddf_i0_error":0.04,"guinier_i0":150.78,"guinier_i0_error":0.072,"pddf_rg":2.77,"pddf_rg_error":0.008,"guinier_rg":2.71,"guinier_rg_error":0.02,"pddf_dmax":8.15,"pddf_dmax_error":null,"porod_volume":118.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":45,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBT4_datloglog_img.png","symmetry":null,"last_modified":"2020-08-17T10:26:30.598958+02:00","bragg_peak":[]}