{"code":"SASDBU4","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Domain Organization of Vaccinia Virus Helicase-Primase D5.","publication":{"title":"Domain Organization of Vaccinia Virus Helicase-Primase D5.","author_list":"Hutin S, Ling WL, Round A, Effantin G, Reich S, Iseni F, Tarbouriech N, Schoehn G, Burmeister WP","journal":"J Virol","doi":"10.1128/JVI.00044-16","pmid":"26912611","published_date":"2016 May"},"status":"released","submitted_date":"2016-04-11","released_date":"2016-04-11"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBU4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBU4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBU4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBU4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBU4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBU4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBU4.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray 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virus","uniprot_code":"P21010","uniprot_range_first":323,"uniprot_range_last":785,"oligomerization":"hexamer","molecular_type":"protein","uniprot_sequence":"MDAAIRGNDVIFVLKTIGVPSACRQNEDPRFVEAFKCDELKRYIDNNPECTLFESLRDEE\nAYSIVRIFMDVDLDACLDEIDYLTAIQDFIIEVSNCVARFAFTECGAIHENVIKSMRSNF\nSLTKSTNRDKTSFHIIFLDTYTTMDTLIAMKRTLLELSRSSENPLTRSIDTAVYRRKTTL\nRVVGTRKNPNCDTIHVMQPPHDNIEDYLFTYVDMNNNSYYFSLQRRLEDLVPDKLWEPGF\nISFEDAIKRVSKIFINSIINFNDLDENNFTTVPLVIDYVTPCALCKKRSHKHPHQLSLEN\nGAIRIYKTGNPHSCKVKIVPLDGNKLFNIAQRILDTNSVLLTERGDYIVWINNSWKFNSE\nEPLITKLILSIRHQLPKEYSSELLCPRKRKTVEANIRDMLVDSVETDTYPDKLPFKNGVL\nDLVDGMFYSGDDAKKYTCTVSTGFKFDDTKFVEDSPEMEELMNIINDIQPLTDENKKNRE\nLYEKTLSSCLCGATKGCLTFFFGETATGKSTTKRLLKSAIGDLFVETGQTILTDVLDKGP\nNPFIANMHLKRSVFCSELPDFACSGSKKIRSDNIKKLTEPCVIGRPCFSNKINNRNHATI\nIIDTNYKPVFDRIDNALMRRIAVVRFRTHFSQPSGREAAENNDAYDKVKLLDEGLDGKIQ\nNNRYRFAFLYLLVKWYKKYHVPIMKLYPTPEEIPDFAFYLKIGTLLVSSSVKHIPLMTDL\nSKKGYILYDNVVTLPLTTFQQKISKYFNSRLFGHDIESFINRHKKFANVSDEYLQYIFIE\nDISSP","mw":53.48,"total_mw":320.879,"number_molecules":6,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 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GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from solutions of the primase D5 protein fragment from Vaccinia virus containing the D5N and helicase domain in 20 mM Tris 150 mM NaCL, 5% glycerol, 1mM DTT pH 7 on the BM29 SAXS beam line at the ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.1 nm). A solute concentration of 1.42 mg/ml was measured using 2000 successive 2 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted and the different curves were scaled and averaged.","tags":[],"intensity_unit":null,"experimental_mw":345.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":29.0,"pddf_i0_error":0.04,"guinier_i0":29.5,"guinier_i0_error":0.04,"pddf_rg":4.7,"pddf_rg_error":0.006,"guinier_rg":4.8,"guinier_rg_error":0.03,"pddf_dmax":14.5,"pddf_dmax_error":null,"porod_volume":570.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":2,"guinier_point_last":24,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBU4_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}