{"code":"SASDBU9","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Human βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study.","publication":{"title":"Human βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study.","author_list":"Xi Z, Whitley MJ, Gronenborn AM","journal":"Structure","doi":"10.1016/j.str.2017.02.001","pmid":"28238532","published_date":"2017 Mar 7"},"status":"released","submitted_date":"2017-01-17","released_date":"2017-03-21"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBU9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBU9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBU9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBU9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBU9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBU9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBU9.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Advanced Photon Source (APS), Argonne National Laboratory","city":"Lemont, IL","country":"USA","beamline_name":"12-ID-B SAXS/WAXS","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Beta-crystallin B2","short_name":null,"sequence":">|terminally truncated human βB2-crystallin\r\nMLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAPHPQVQSVRRIRDMQW","organism":"Homo sapiens","uniprot_code":"P43320","uniprot_range_first":15,"uniprot_range_last":195,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWV\nGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGK\nKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAP\nHPQVQSVRRIRDMQWHQRGAFHPSN","mw":20.911,"total_mw":41.822,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM NaPi, 5 mM DTT, 1 mM EDTA,","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.5,"deuteration":null},"purity_method":null,"name":"Terminally truncated human βB2-crystallin","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Pittsburgh, PA USA","full_name":"University of Pittsburgh","webpage":"http://www.pitt.edu/"}],"contributor_name":"Zhaoyong","contributor_surname":"Xi","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2015-03-20","storage_temperature":null,"cell_temperature":null,"exposure_time":0.4,"number_of_frames":30,"wavelength":0.08856,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":1.75,"sample_volume":null,"flow_rate":null,"s_min":0.323,"s_max":4.499,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBU9_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBU9_fit1_model1.pdb","model_mw":31.585,"bead_radius":2.0,"log":null,"symmetry":"","comment":"","user":200}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBU9_fit1_fit_img.png","software":"DAMMIN","chi_square_value":0.216,"p_value":0.0384,"fit_residual_plot":"SASDBU9_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBU9_fit1.fir","fit_log":null,"software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"ATSAS 2.6.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of truncated human βB2-crystallin in 25 mM sodium phosphate, 5 mM DTT, 1 mM EDTA, pH 6.5, were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS, Argonne, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.08856 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Data were acquired over 30 successive 0.400 second frames from a sample at 1.75 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the radially-averaged solvent-blank was subtracted to produce the scattering profile displayed in this entry.","tags":[],"intensity_unit":null,"experimental_mw":38.6,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":38.6,"porod_mw_error":null,"pddf_i0":0.0513,"pddf_i0_error":0.0002,"guinier_i0":0.051,"guinier_i0_error":0.0004,"pddf_rg":2.076,"pddf_rg_error":0.012,"guinier_rg":2.09,"guinier_rg_error":0.019,"pddf_dmax":6.7,"pddf_dmax_error":null,"porod_volume":61.84,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":2,"guinier_point_last":29,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBU9_datloglog_img.png","symmetry":null,"last_modified":"2023-03-02T16:54:16.648202+01:00","bragg_peak":[]}