{"code":"SASDBY6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural and functional studies of the Leishmania braziliensis mitochondrial Hsp70: Similarities and dissimilarities to human orthologues.","publication":{"title":"Structural and functional studies of the Leishmania braziliensis mitochondrial Hsp70: Similarities and dissimilarities to human orthologues.","author_list":"Dores-Silva PR, Nishimura LS, Kiraly VT, Borges JC","journal":"Arch Biochem Biophys","doi":"10.1016/j.abb.2016.11.004","pmid":"27840097","published_date":"2017 Jan 1"},"status":"released","submitted_date":"2016-09-27","released_date":"2016-11-17"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBY6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBY6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBY6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBY6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBY6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBY6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBY6.sascif","experiment":{"instrument":{"detector":{"type":"20Hz","name":"Pilatus 300K","resolution":null},"name":"Brazilian Synchrotron Light Laboratory","city":"Campinas","country":"Brazil","beamline_name":"SAXS1 Beamline","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Mitochondrial heat shock protein 70","short_name":"LbmtHsp70","sequence":"MGSSHHHHHHSSGLVPRGSHMESQKVTGDVIGVDLGTTYSCVATMDGDKARVLENSEGFRTTPSVVAFKGSEKLVGLAAKRQAITNPQSTFYAVKRLIGRRFEDEHIQRDIKNVPYKIVRAGNGDAWVQDGNGKQYSPSQVGAFVLEKMKETAQNFLGHTVSNAVVTCPAYFNDAQRQATKDAGTIAGLNVIRVVNEPTAAALAYGMDKTKDSLIAVYDLGGGTFDISVLEIAGGVFEVKATNGDTHLGGEDFDLALSDYILEEFRKTSGIDLSKERMALQRVREAAEKAKCELSSAMETEVNLPFITANADGAQHIQMHISRSKFEGITTRLIERSIAPCKQCIKDAGVELKEINDVVLVGGMTRMPKVVEEVKRFFQKEPFRGVNPDEAVALGAATLGGVLRGDVKGLVLLDVTPLSLGIETLGGVFTRMIPKNTTIPTKKSQTFSTAADNQTQVGIKVFQGEREMAADNQMMGQFDLVGIPPAPRGVPQVEVTFDIDANGICHVTAKDKATGKTQNITITAHGGLSKEQIEQMVRDSEQHAEADRVKRELVEARNNAETQLTTAERQLGEWKYVSDAEKENVKTHVAELRKAMENPNVAKDDLVAATDKLQKAVMECGRTEYQQAAAANSGSSSNSGEQQQQQQQSSEKN","organism":"Leishmania braziliensis","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":null,"mw":70.902,"total_mw":70.902,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris-HCl, 50 mM NacL, 5 mM KCl, 5 mM sodium phosphate, 2 mM B-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Leishmania braziliensis Mitochondrial heat shock protein 70 (LbmtHSP70)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Júlio","contributor_surname":"Borges","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2012-06-22","storage_temperature":4.0,"cell_temperature":25.0,"exposure_time":60.0,"number_of_frames":5,"wavelength":0.1488,"sample_detector_distance":1.0,"concentration_min":null,"concentration_max":0.5,"sample_volume":null,"flow_rate":null,"s_min":0.193,"s_max":3.508,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBY6_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBY6_fit1_model1.pdb","model_mw":67.75,"bead_radius":3.25,"log":"https://www.sasbdb.org/media/log_files/damsup.log","symmetry":"","comment":"","user":161}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBY6_fit1_fit_img.png","software":null,"chi_square_value":1.507,"p_value":0.401,"fit_residual_plot":"SASDBY6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBY6_fit1.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"GNOM - Version 4.5a","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Leishmania braziliensis mitochondrial heat shock protein 70 (LbmtHSP70) in 25 mM Tris-HCl, 50 mM NaCL, 5 mM KCl, 5 mM sodium phosphate, 2 mM β-mercaptoethanol, pH 7.5, were collected on the SAXS1 Beamline at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a 20Hz Pilatus 300K detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.1488 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Scattering data from solute concentration of 0.5 mg/ml was measured at 25°C. Five successive 60 second data frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the radially-averaged scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":null,"experimental_mw":76.0,"experimental_mw_error":null,"guinier_i0_mw":76.0,"guinier_i0_mw_error":8.0,"porod_mw":74.0,"porod_mw_error":null,"pddf_i0":0.02592,"pddf_i0_error":0.0001413,"guinier_i0":0.025262101,"guinier_i0_error":0.0025,"pddf_rg":3.779,"pddf_rg_error":0.032,"guinier_rg":3.63,"guinier_rg_error":0.2,"pddf_dmax":14.0,"pddf_dmax_error":1.0,"porod_volume":118.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":14,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBY6_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}