{"code":"SASDBZ4","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Stable preparations of tyrosine hydroxylase provide the solution structure of the full-length enzyme.","publication":{"title":"Stable preparations of tyrosine hydroxylase provide the solution structure of the full-length enzyme.","author_list":"Bezem MT, Baumann A, Skjærven L, Meyer R, Kursula P, Martinez A, Flydal MI","journal":"Sci Rep","doi":"10.1038/srep30390","pmid":"27462005","published_date":"2016 Jul 27"},"status":"released","submitted_date":"2016-06-25","released_date":"2018-01-23"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBZ4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBZ4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBZ4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBZ4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBZ4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBZ4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBZ4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Tyrosine hydroxylase, isoform 1","short_name":"TYH","sequence":"MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAV\r\nPSEPGDPLEAVAFEEKEGKAVLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQR\r\nPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLV\r\nTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLK\r\nGLYATHACGEHLEAFALLERFSGYREDNIPQLEDVSRFLKERTGFQLRPVAGLLSARDFL\r\nASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASD\r\nEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQ\r\nPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLE\r\nGVQDELDTLAHALSAIG","organism":"Homo sapiens","uniprot_code":"P07101","uniprot_range_first":1,"uniprot_range_last":497,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MPTPDATTPQAKGFRRAVSELDAKQAEAIMVRGQGAPGPSLTGSPWPGTAAPAASYTPTP\nRSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAVLNLLFSPR\nATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVR\nQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEI\nAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNI\nPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCC\nHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLYWFTVEFGLCKQNGEVKAYG\nAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYAS\nRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHALSAIG","mw":55.611,"total_mw":222.445,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Na-HEPES 200 mM NaCl","concentration_unit":"mM","comment":null,"additive":"200 mM NaCl","concentration":20.0,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Tyrosine hydroxylase (Isoform 1, Homo sapiens)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"UiB","address":"Bergen, Norway","full_name":"University of Bergen","webpage":"http://www.uib.no"}],"contributor_name":"Lars","contributor_surname":"Skjærven","orcid":null}],"concentration_method":null,"concentration_unit":"mg/ml","date":"2015-06-14","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.12,"sample_detector_distance":3.1,"concentration_min":1.0,"concentration_max":3.0,"sample_volume":null,"flow_rate":null,"s_min":0.027,"s_max":4.801,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBZ4_fit1_model1_img.png","software":"BUNCH","pdb_link":[{"pdb_code":"1toh","ext_code":"1TOH","ext_type":"pdb","difference_with_model":"derived"},{"pdb_code":"2mda","ext_code":"2MDA","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDBZ4_fit1_model1.pdb","model_mw":222.8,"bead_radius":1.9,"log":null,"symmetry":"P222","comment":null,"user":134}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBZ4_fit1_fit_img.png","software":"CRYSOL","chi_square_value":1.55,"p_value":0.0,"fit_residual_plot":"SASDBZ4_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBZ4_fit1.fit","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from solutions of tyrosine hydroxylase, isoform 1 in 20 mM Na-HEPES, 200 mM NaCl, pH 7.0 were collected on the P12 beam line of Petra-III (Hamburg, Germany) using a Pilatus 2M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.124 nm). Different solute concentrations in the range 1.0-2.0 mg/ml were measured using an exposure time of 1 s (recorded as 20 x 0.050 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data presented here are merged SAXS data derived from the solute concentration series.","tags":[],"intensity_unit":null,"experimental_mw":283.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":63330.0,"pddf_i0_error":219.0,"guinier_i0":62494.4,"guinier_i0_error":124.65,"pddf_rg":4.93,"pddf_rg_error":0.03,"guinier_rg":4.74,"guinier_rg_error":0.33,"pddf_dmax":20.0,"pddf_dmax_error":null,"porod_volume":519.8,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":40,"guinier_point_last":95,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBZ4_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}