{"code":"SASDBZ5","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structure of the NPr:EIN<sup>Ntr</sup> Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems.","publication":{"title":"Structure of the NPr:EIN<sup>Ntr</sup> Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems.","author_list":"Strickland M, Stanley AM, Wang G, Botos I, Schwieters CD, Buchanan SK, Peterkofsky A, Tjandra N","journal":"Structure","doi":"10.1016/j.str.2016.10.007","pmid":"27839951","published_date":"2016 Dec 6"},"status":"released","submitted_date":"2016-08-29","released_date":"2016-11-11"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDBZ5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDBZ5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBZ5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBZ5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBZ5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBZ5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDBZ5.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"NIDDK, NIH","city":"Bethesda, MD","country":"USA","beamline_name":"Rigaku BioSAXS-1000","beam_geometry":"","type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Phosphocarrier protein NPr","short_name":"NPr","sequence":"MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKG\r\nRQIEVEATGPQEEEALAAVIALFNS","organism":"Escherichia coli","uniprot_code":"P0A9N0","uniprot_range_first":1,"uniprot_range_last":85,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKG\nRQIEVEATGPQEEEALAAVIALFNSGFDED","mw":9.25,"total_mw":9.25,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Phosphoenolpyruvate-protein phosphotransferase PtsP","short_name":"Enzyme I-Ntr","sequence":"RIRALPAAPGV\r\nAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFAAGAQKETAA\r\nIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALSDNYLKERAGDLRA\r\nLGQRLLFHLDDANQGPNAWPERFILVADELSATTLAELPQDRLVGVVVRDGAANSHAAIM\r\nVRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDPEPVLLQEYQRLISEEIELSRLAE\r\nDDVN","organism":"Escherichia coli","uniprot_code":"P37177","uniprot_range_first":170,"uniprot_range_last":424,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKK\nPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQ\nLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLTALFGQYRQTRIRALPAAPGV\nAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFAAGAQKETAA\nIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALSDNYLKERAGDLRA\nLGQRLLFHLDDANQGPNAWPERFILVADELSATTLAELPQDRLVGVVVRDGAANSHAAIM\nVRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDPEPVLLQEYQRLISEEIELSRLAE\nDDVNLPAQLKSGERIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV\nAQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR\nAMLRANAATGNLNILLPMVTSLDEVDEARRLIERAGREVEEMIGYEIPKPRIGIMLEVPS\nMVFMLPHLAKRVDFISVGTNDLTQYILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEI\nHGIDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARAKYLLRRIDYAEAENLAQRSLE\nAQLATEVRHQVAAFMERRGMGGLIRGGL","mw":28.35,"total_mw":28.35,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"10 mM Tris 150 mM NaCl 0.5 mM EDTA","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Enzyme I-Ntr (residues 170-424) in complex with NPr (residues 1-85)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"NIH","address":"Bethesda, Maryland, USA","full_name":"National Institutes of Health, Bethesda, MD","webpage":"https://www.nih.gov/"}],"contributor_name":"Madeleine","contributor_surname":"Strickland","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-01-30","storage_temperature":4.0,"cell_temperature":27.0,"exposure_time":1800.0,"number_of_frames":8,"wavelength":0.154,"sample_detector_distance":null,"concentration_min":null,"concentration_max":3.76,"sample_volume":null,"flow_rate":null,"s_min":0.181,"s_max":6.797,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDBZ5_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"5t1o","ext_code":"5t1o","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":"2.8.3","model_data":"https://www.sasbdb.org/media/pdb_file/SASDBZ5_fit1_model1.pdb","model_mw":37.52,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/5t1o-100.log","symmetry":"","comment":"Fig. 5J","user":154}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBZ5_fit1_fit_img.png","software":null,"chi_square_value":1.25,"p_value":7.8e-05,"fit_residual_plot":"SASDBZ5_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDBZ5_fit1.fit","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"ATSAS Primus","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of Enzyme I-Ntr (residues 170-424) complexed with NPr (residues 1-85) in 10 mM Tris 150 mM NaCl 0.5 mM EDTA, pH 7.5 were collected using an in house X-ray source at the NIDDK, National Institutes of Health, Bethesda, MD using a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4π sin θ/λ, and 2θ is the scattering angle). One solute concentration of 3.76 mg/ml was measured. Eight successive 1800 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a single concentration scattering curve.","tags":[],"intensity_unit":null,"experimental_mw":37.6,"experimental_mw_error":null,"guinier_i0_mw":22.0,"guinier_i0_mw_error":null,"porod_mw":30.6,"porod_mw_error":null,"pddf_i0":2.87,"pddf_i0_error":null,"guinier_i0":2.83,"guinier_i0_error":null,"pddf_rg":2.373,"pddf_rg_error":null,"guinier_rg":2.319,"guinier_rg_error":null,"pddf_dmax":8.16,"pddf_dmax_error":null,"porod_volume":52.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":7,"guinier_point_last":25,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDBZ5_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}