{"code":"SASDC22","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans.","publication":{"title":"Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans.","author_list":"Bhati M, Llamosas E, Jacques DA, Jeffries CM, Dastmalchi S, Ripin N, Nicholas HR, Matthews JM","journal":"Sci Rep","doi":"10.1038/s41598-017-04587-8","pmid":"28676648","published_date":"2017 Jul 4"},"status":"released","submitted_date":null,"released_date":"2017-07-08"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC22.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC22.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC22_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC22_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC22_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC22_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC22.sascif","experiment":{"instrument":{"detector":{"type":"KAF 2084 x 2084 SCX CCD","name":"Roper Scientific PI-SCX:4300","resolution":24.0},"name":"University of Sydney","city":"Sydney","country":"Australia","beamline_name":"Anton Paar SAXSess","beam_geometry":"Line collimation","type_of_source":"X-ray in house","point_source":false,"line_collimation":true,"sample_path_length":1.0,"line_collimation_slitlength":10.0,"line_collimation_integrationwidth":10.0,"xray_energy":null,"beam_profile_ah":2.8,"beam_profile_al":1.3},"sample":{"molecule":[{"long_name":"Homeobox protein CEH-14","short_name":"CEH-14","sequence":"GSNNEEAICSLCDKKIRDRFVSKVNGRCYHSSCLRCSTCKDELGATCFLREDSMYCRAHF \r\nYKKFGTKCSSCNEGIVPDHVVRKASNHVYHVECFQCFICKRSLETGEEFYLIADDARLVC \r\nKDDYEQARDGGSGGHMGSGG","organism":"Caenorhabditis elegans","uniprot_code":"P20271","uniprot_range_first":42,"uniprot_range_last":168,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MLGHNILTLGECDELDNHIVMCSTGLLSPQEDFSNVNAGHPNNEEAICSLCDKKIRDRFV\nSKVNGRCYHSSCLRCSTCKDELGATCFLREDSMYCRAHFYKKFGTKCSSCNEGIVPDHVV\nRKASNHVYHVECFQCFICKRSLETGEEFYLIADDARLVCKDDYEQARDKHCNELEGDGSN\nKRPRTTISAKSLETLKQAYQTSSKPARHVREQLASETGLDMRVVQVWFQNRRAKEKRLKK\nDAGRRWKSSNRAESDSNSPIESINGQSPNYLYLDHPMDDGNESNYLFHSREQTPDKYYRN\nETPSTDPPPMHMTTPSVLTTNFSTPLSLSTNVYNLPPPESQLIPHMTPQYI","mw":15.693,"total_mw":15.693,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"CeLIM-7","short_name":"CeLIM-7","sequence":"GIGPLMVQPATPHIDNTLGGPIDIQHFAQWEF","organism":"Caenorhabditis elegans","uniprot_code":"G5EC36","uniprot_range_first":347,"uniprot_range_last":376,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MNICMRNGYEQFSLTSPGTSDLEIGGSFWKDEPDTKYLCLDSPVEQRQHQPPMAVCAGCR\nLEISDRYFLRVNPNLEFHAQCLKCVQCSRPLDENQTAFVKNGQTYCRDDYRRLFTTRCSR\nCHGDFDKTDLVMRAGPQNVFHLNCFACVACEKRLQTGEEFQIKNNSLYCRSDCRGLDNPD\nTSASVPDYSKLNNNNNNDNNNSSSNFDEDEWDEERSTLTSLDNNTSSPLGSPKSDGVRTP\nLFGHHNSGSGGSTSSCGKKKKDKQATRVRTVLNENQLKILRDCYSINSRPDATLKERLVE\nMTGLSARVIRVWFQNKRCKDKKRQIQITENRLNSEREEVLNRVRVNGIGPLMVQPATPHI\nDNTLGGPIDIQHFAQWNGTPPPPPPQYGNPMMFNSPSTFDVSVILAPVAPNVTSPSEALG\nPLGASVFPHFSPQHAPFTATSHDISSPAPCGE","mw":3.501,"total_mw":3.501,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Tris, 100 mM NaCl, 5 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Tandem LIM domains of the neuronal transcription factor homeobox protein CEH-14 fused to the LIM interaction domain of ceLIM-7","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Cy M","contributor_surname":"Jeffries","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2009-04-07","storage_temperature":null,"cell_temperature":10.0,"exposure_time":900.0,"number_of_frames":4,"wavelength":0.1542,"sample_detector_distance":0.309,"concentration_min":null,"concentration_max":5.2,"sample_volume":null,"flow_rate":null,"s_min":0.067,"s_max":3.311,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC22_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC22_fit1_model1.pdb","model_mw":17.376,"bead_radius":2.25,"log":null,"symmetry":"","comment":"","user":9},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC22_fit1_model2_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC22_fit1_model2.pdb","model_mw":17.473,"bead_radius":2.25,"log":null,"symmetry":"","comment":"","user":9}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC22_fit1_fit_img.png","software":"DAMMIF","chi_square_value":0.288,"p_value":0.1902,"fit_residual_plot":"SASDC22_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC22_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/refine_hdm80LL.log","software_version":"","description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC22_fit2_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC22_fit2_model1.pdb","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":9}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC22_fit2_fit_img.png","software":"GASBOR","chi_square_value":0.29,"p_value":0.0257,"fit_residual_plot":"SASDC22_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC22_fit2.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/Ceh5.log","software_version":"","description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC22_fit3_model1_img.png","software":"SwissModel","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC22_fit3_model1.pdb","model_mw":18.77,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":9}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC22_fit3_fit_img.png","software":"CRYSOL","chi_square_value":0.3,"p_value":0.192,"fit_residual_plot":"SASDC22_fit3_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC22_fit3.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/model_ceh14_lim7r_Znr00.log","software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"GNOM5 (r8972)","i0_calibration_standard":null,"description":"The SAXS data displayed for this entry includes the beam-geometry corrected scattering profile (desmeared data, top, and homology model fit) as well as the fit of the ab initio models to the smeared SAXS data (i.e., smeared model-data fits). The original smeared data, prepared experimental beam profile and all individual DAMMIF and Gasbor models are included in the full entry zip archive.","experiment_description":"SAXS data from solutions of the tandem LIM domains of the neuronal transcription factor homeobox protein CEH-14 fused to the LIM interaction domain of ceLIM-7 in 50 mM Tris, 100 mM NaCl, 5 mM TCEP, pH 8 were collected on an Anton Paar SAXSess 10 mm line collimated instrument (The University of Sydney, Australia) using a CCD Roper Scientific KAF 2084 x 2084 SCX CCD camera at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.1542 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 5.50 mg/ml was measured at 10°C. Four successive 900 second frames were collected. The data were normalized to the intensity of the transmitted beam and underwent 10 mm horizontal-width integration; the scattering of the solvent-blank was subtracted to produce the smeared scattering profile of the protein in solution.","tags":[],"intensity_unit":null,"experimental_mw":17.0,"experimental_mw_error":null,"guinier_i0_mw":16.7,"guinier_i0_mw_error":0.9,"porod_mw":16.0,"porod_mw_error":null,"pddf_i0":0.0833,"pddf_i0_error":0.002,"guinier_i0":0.083,"guinier_i0_error":0.0002,"pddf_rg":2.51,"pddf_rg_error":0.09,"guinier_rg":2.4,"guinier_rg_error":0.03,"pddf_dmax":8.9,"pddf_dmax_error":null,"porod_volume":25.6,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":61,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC22_datloglog_img.png","symmetry":null,"last_modified":"2020-01-10T16:55:24.317339+01:00","bragg_peak":[]}