{"code":"SASDC38","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"The atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase.","publication":{"title":"The atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase.","author_list":"Walden PM, Whitten AE, Premkumar L, Halili MA, Heras B, King GJ, Martin JL","journal":"Acta Crystallogr D Struct Biol","doi":"10.1107/S2059798318018442","pmid":"30950399","published_date":"2019 Mar 1"},"status":"released","submitted_date":"2017-10-03","released_date":"2019-03-29"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC38.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC38.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC38_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC38_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC38_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC38_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC38.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Australian  Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":"Point","type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"DsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein)","short_name":"α-DsbA2","sequence":"SNAARDNVTKSKISQYKDQIFDLTYPYSGNENSSVIAVGFLDYSCGHCKAIKNDIKQLINDGKIKYIFRDAPILGNASLKAAKSALAVYFLNKEKYFDFHHAALSHKGEFSDESILDIVKNIGIDEDDFNDSIKDNADKIEQMINNSR\r\nLLVRDLGVGGTPFLIIGDSLFVGATDLNVLRKKVDELSHKQG","organism":"Wolbachia endosymbiont of Drosophila 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NSW 2232, Australia","full_name":"Australian Nuclear Science and Technology Organisation","webpage":""}],"contributor_name":"Andrew","contributor_surname":"Whitten","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2012-02-29","storage_temperature":4.0,"cell_temperature":10.0,"exposure_time":2.0,"number_of_frames":8,"wavelength":0.1033,"sample_detector_distance":1.575,"concentration_min":1.25,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.1,"s_max":3.996,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC38_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"5.3","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC38_fit1_model1.pdb","model_mw":19.264,"bead_radius":1.75,"log":"https://www.sasbdb.org/media/log_files/damsel_sJi2sTc.log","symmetry":"P1","comment":"Filtered model from Damaver","user":111}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC38_fit1_fixed_fit_img.png","software":"DAMMIN","chi_square_value":1.034,"p_value":0.247123,"fit_residual_plot":"SASDC38_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC38_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/run0_FAZDfOb.log","software_version":"5.3","description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC38_fit2_model1_img.png","software":"CORAL","pdb_link":[{"pdb_code":"6eez","ext_code":"6eez","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":"1.1","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC38_fit2_model1.pdb","model_mw":21.13,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/run8.log","symmetry":"P1","comment":"","user":111}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC38_fit2_fixed_fit_img.png","software":"CORAL","chi_square_value":3.88,"p_value":0.0,"fit_residual_plot":"SASDC38_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC38_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/run8.log","software_version":"1.1","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"X-ray synchrotron radiation scattering data from the truncated version of α-DsbA2 from Wolbachia pipientis in 25 mM TRIS 150mM NaCl pH 7.5 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Eight successive 2 second frames were collected across solute concentrations of 1.25-5.00 mg/ml. The SAXS data displayed this entry was derived from a 1.25 mg/ml sample. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The models and corresponding fits include those derived from dummy-atom modelling using DAMMIN (top) and rigid-body modelling using CORAL (bottom).","tags":[],"intensity_unit":"1/cm","experimental_mw":23.0,"experimental_mw_error":null,"guinier_i0_mw":23.0,"guinier_i0_mw_error":1.0,"porod_mw":23.0,"porod_mw_error":null,"pddf_i0":0.0215,"pddf_i0_error":0.0001,"guinier_i0":0.0215,"guinier_i0_error":0.0001,"pddf_rg":1.88,"pddf_rg_error":0.01,"guinier_rg":1.87,"guinier_rg_error":0.01,"pddf_dmax":6.3,"pddf_dmax_error":0.3,"porod_volume":275.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":45,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC38_datloglog_img.png","symmetry":null,"last_modified":"2021-04-10T09:53:41.823307+02:00","bragg_peak":[]}