{"code":"SASDC53","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N.","publication":{"title":"The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N.","author_list":"Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH","journal":"Biophys J","doi":"10.1016/j.bpj.2017.08.030","pmid":"29045862","published_date":"2017 Oct 17"},"status":"released","submitted_date":"2017-04-26","released_date":"2017-10-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC53.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC53.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC53_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC53_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC53_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC53_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC53.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Colicin N Translocation domain","short_name":"ColN-T","sequence":"MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWSWSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASSHHHHHH","organism":"Escherichia coli","uniprot_code":"P08083","uniprot_range_first":1,"uniprot_range_last":90,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWSWSNKPHKNDGFHSDG\nSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNSKPGRYISSNPEYSLLA\nKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNWKGPKYNNKLVKRFVSQF\nLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGVKYKNVAKEVANDIKNFHGRNIRSY\nNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAKDMANKIGNLGKAFKVADLAIKVEK\nIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGAVLSFLPISGLAVTALGVIGI\nMTISYLSSFIDANRVSNINNIISSVIR","mw":9.965,"total_mw":9.965,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Na-Phosphate 300 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.6,"deuteration":null},"purity_method":null,"name":"Colicin N Translocation domain","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Newcastle, UK","full_name":"Newcastle University","webpage":"http://www.ncl.ac.uk"}],"contributor_name":"Alex","contributor_surname":"Solovyova","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2012-06-29","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":2.0,"number_of_frames":10,"wavelength":0.15,"sample_detector_distance":2.85,"concentration_min":0.4,"concentration_max":6.3,"sample_volume":null,"flow_rate":null,"s_min":0.032,"s_max":4.548,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC53_fit1_model1_img.png","software":"EOM/GAJOE","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC53_fit1_model1.pdb","model_mw":10.04,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/GA003.log","symmetry":"","comment":"","user":213},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC53_fit1_model2_img.png","software":"EOM/GAJOE","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC53_fit1_model2.pdb","model_mw":9.949,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/GA003_9tEwzuh.log","symmetry":"","comment":"","user":213}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC53_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.5,"p_value":0.000494,"fit_residual_plot":"fit4columns817_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC53_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/GA003.log","software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The results obtained from the bayesapp estimation of distribution functions from small-angle scattering data (http://www.bayesapp.org/) are included in the full entry zip archive.","experiment_description":"Synchrotron SAXS data from solutions of Colicin N Translocation domain in 50 mM Na-Phosphate 300 mM NaCl, pH 7.6 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 6.3 mg/ml were measured at 4°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.","tags":["idp"],"intensity_unit":null,"experimental_mw":10.0,"experimental_mw_error":null,"guinier_i0_mw":18.5,"guinier_i0_mw_error":0.88,"porod_mw":null,"porod_mw_error":null,"pddf_i0":23.4,"pddf_i0_error":0.0777,"guinier_i0":22.95,"guinier_i0_error":0.044,"pddf_rg":2.93,"pddf_rg_error":0.02,"guinier_rg":2.76,"guinier_rg_error":0.15,"pddf_dmax":11.42,"pddf_dmax_error":0.25,"porod_volume":21.82,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":53,"guinier_point_last":110,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC53_datloglog_img.png","symmetry":null,"last_modified":"2021-08-18T17:26:22.657806+02:00","bragg_peak":[]}