{"code":"SASDC62","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it.","publication":{"title":"Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it.","author_list":"Necasová I, Janoušková E, Klumpler T, Hofr C","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkx812","pmid":"28981702","published_date":"2017 Dec 1"},"status":"released","submitted_date":"2017-01-06","released_date":"2017-09-18"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC62.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC62.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC62_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC62_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC62_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC62_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC62.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"CEITEC","city":"Brno","country":"Czech Republic","beamline_name":"Rigaku BioSAXS-1000","beam_geometry":"","type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Basic domain of telomeric repeat-binding factor 2","short_name":"TRF2","sequence":"GPPGSMAGGGGSSDGSGRAAGRRASRSSGRARRGRHEPGLGGPAERGAG","organism":"Homo sapiens","uniprot_code":"Q15554","uniprot_range_first":42,"uniprot_range_last":86,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MAAGAGTAGPASGPGVVRDPAASQPRKRPGREGGEGARRSDTMAGGGGSSDGSGRAAGRR\nASRSSGRARRGRHEPGLGGPAERGAGEARLEEAVNRWVLKFYFHEALRAFRGSRYGDFRQ\nIRDIMQALLVRPLGKEHTVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEM\nIKTEFTLTEAVVESSRKLVKEAAVIICIKNKEFEKASKILKKHMSKDPTTQKLRNDLLNI\nIREKNLAHPVIQNFSYETFQQKMLRFLESHLDDAEPYLLTMAKKALKSESAASSTGKEDK\nQPAPGPVEKPPREPARQLRNPPTTIGMMTLKAAFKTLSGAQDSEAAFAKLDQKDLVLPTQ\nALPASPALKNKRPRKDENESSAPADGEGGSELQPKNKRMTISRLVLEEDSQSTEPSAGLN\nSSQEAASAPPSKPTVLNQPLPGEKNPKVPKGKWNSSNGVEEKETWVEEDELFQVQAAPDE\nDSTTNITKKQKWTVEESEWVKAGVQKYGEGNWAAISKNYPFVNRTAVMIKDRWRTMKRLG\nMN","mw":4.587,"total_mw":4.587,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM NaPi, 50 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Basic domain of telomeric repeat-binding factor 2 (TRF2)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"CEITEC - Central European Institute of Technology, Masaryk University","webpage":"https://www.ceitec.eu/"}],"contributor_name":"Tomas","contributor_surname":"Klumpler","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-03-05","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":3600.0,"number_of_frames":6,"wavelength":1.54,"sample_detector_distance":0.48,"concentration_min":null,"concentration_max":1.2,"sample_volume":null,"flow_rate":null,"s_min":0.109,"s_max":6.503,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"primus/qt r8972","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of the basic domain of telomeric repeat-binding factor 2 (TRF2) in 50 mM sodium phosphate, 50 mM NaCl, pH 7, were collected using a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m (I(s) vs s, where = 4π sin θ/λ; 2θ is the scattering angle and λ = 0.154 nm). Six successive 3600 second frames were collected at a sample temperature of 4°C using a solute concentration of 1.2 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged and the corresponding scattering from the solvent-blank was subtracted to produced the scattering profile displayed in this entry.","tags":["idp"],"intensity_unit":null,"experimental_mw":4.587,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":2.3,"porod_mw_error":null,"pddf_i0":0.106,"pddf_i0_error":0.001,"guinier_i0":0.103287,"guinier_i0_error":0.000861426,"pddf_rg":1.819,"pddf_rg_error":0.03,"guinier_rg":1.657,"guinier_rg_error":0.168,"pddf_dmax":7.06,"pddf_dmax_error":null,"porod_volume":3.69,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":6,"guinier_point_last":48,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC62_datloglog_img.png","symmetry":null,"last_modified":"2022-03-31T22:26:26.370859+02:00","bragg_peak":[]}