{"code":"SASDC63","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039.","publication":{"title":"Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039.","author_list":"Anasir MI, Caria S, Skinner MA, Kvansakul M","journal":"J Biol Chem","doi":"10.1074/jbc.M116.768879","pmid":"28411240","published_date":"2017 Jun 2"},"status":"released","submitted_date":"2017-04-28","released_date":"2017-04-29"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC63.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC63_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC63_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC63_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC63.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Australian  Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":"Point","type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Bcl-2-like protein FPV039","short_name":"FPV039","sequence":"MASSNMKDETYYIALNMIQNYIIEYNTNKPRKSFVIDSISYDVLKAACKSVIKTNYNEFDIIISRNIDFNVIVTQVLEDKINWGRIITIIAFCAYYSKKVKQDTSPQYYDGIISEAITDAILSKYRSWFIDQDYWNGIRIYKN","organism":"Fowlpox virus","uniprot_code":"Q9J5G4","uniprot_range_first":1,"uniprot_range_last":143,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MASSNMKDETYYIALNMIQNYIIEYNTNKPRKSFVIDSISYDVLKAACKSVIKTNYNEFD\nIIISRNIDFNVIVTQVLEDKINWGRIITIIAFCAYYSKKVKQDTSPQYYDGIISEAITDA\nILSKYRSWFIDQDYWNGIRIYKNYSYIFNTASYCIFTASLIIASLAVFKICSFYM","mw":16.823,"total_mw":16.823,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Uncharacterized protein (BAK1)","short_name":"Bak","sequence":"LGSTGSQVGRRLAIIGDDINKRYDAE","organism":"Gallus gallus","uniprot_code":"Q5F404","uniprot_range_first":72,"uniprot_range_last":97,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MEGNEGDPPGAHRRRDSHGRRPSREINAEDQVAQETEEVFRSYTFYRYQQEREEGGAEVP\nMDPEIMEIQQELGSTGSQVGRRLAIIGDDINKRYDAEFRHMLKSLQPTKENAYEYFTTIA\nSSLFDSGINWGRVIALLGFGYCMAIHVYQQGITGFLRRIARYVTEFMLRNRIARWIAQQG\nGWVAALDLDNVYMKYMLVVLALVMVGHLVVRRFFRP","mw":2.805,"total_mw":2.805,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM trisodium citrate pH, 200 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.0,"deuteration":null},"purity_method":null,"name":"Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide (FPV039:BAK)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"ANSTO","address":"Kirrawee DC, NSW 2232, Australia","full_name":"Australian Nuclear Science and Technology Organisation","webpage":""}],"contributor_name":"Sofia","contributor_surname":"Caria","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2015-10-02","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":30,"wavelength":null,"sample_detector_distance":1.6,"concentration_min":0.31,"concentration_max":7.35,"sample_volume":null,"flow_rate":null,"s_min":0.106,"s_max":6.185,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide in 20 mM trisodium citrate, 200 mM NaCl, pH 6.0 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.31 to 7.35 mg/ml were measured. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a single concentration scattering curve (7.35 mg/ml scattering data).","tags":[],"intensity_unit":null,"experimental_mw":17.9,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":0.104031,"guinier_i0_error":0.0002,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":1.96,"guinier_rg_error":0.006,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":38,"guinier_point_last":70,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC63_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}