{"code":"SASDC65","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome.","publication":{"title":"Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome.","author_list":"Silva NSM, Seraphim TV, Minari K, Barbosa LRS, Borges JC","journal":"Int J Biol Macromol","doi":"10.1016/j.ijbiomac.2017.11.161","pmid":"29191421","published_date":"2018 Mar"},"status":"released","submitted_date":"2017-06-17","released_date":"2017-12-05"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC65.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC65.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC65_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC65_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC65_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC65_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC65.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"MAR 165 CCD","resolution":null},"name":"Brazilian Synchrotron Light Laboratory","city":"Campinas","country":"Brazil","beamline_name":"SAXS2 Beamline","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Co-chaperone p23","short_name":"Pfp23B","sequence":"GSHMQKLYPIVLWAQKKECLYLTIELQDIENVKIDLKEDKLYFYGTKDKNEYEFTLNFLKPINVEESKYSTQR\r\nNIKFKIIKKEQERWKTLNNDGKKHWVKCDWNSWVDTDEEDKANDYDDMGMNSFGGMGGMPDMSQFGNMGG\r\nLGNMGGLGNMGGLGNMGGLGNMGGLGNMGGLGNMGGLGNMGGMGDLDFSKLGNMGGDMPNFAGLGGMDQF\r\nKNMPNMNNMNDDDSSSYGDDTSDEEDDDDEEDEDVEVDNKTLDSDKLKDEENKIPDAAVEVQEPVA","organism":"Plasmodium falciparum","uniprot_code":"Q8IKU1","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MPLYPIVLWAQKKECLYLTIELQDIENVKIDLKEDKLYFYGTKDKNEYEFTLNFLKPINV\nEESKYSTQRNIKFKIIKKEQERWKTLNNDGKKHWVKCDWNSWVDTDEEDKANDYDDMGMN\nSFGGMGGMPDMSQFGNMGGLGNMGGLGNMGGLGNMGGLGNMGGLGNMGGLGNMGGLGNMG\nGMGDLDFSKLGNMGGDMPNFAGLGGMDQFKNMPNMNNMNDDDSSSYGDDTSDEEDDDDEE\nDEDVEVDNKTLDSDKLKDEENKIPDAAVEVQEPVA","mw":30.932,"total_mw":30.932,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 1 mM B-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Plasmodium falciparum p23B","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Júlio","contributor_surname":"Borges","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-05-26","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":180.0,"number_of_frames":1,"wavelength":0.1488,"sample_detector_distance":1.0,"concentration_min":2.0,"concentration_max":3.0,"sample_volume":null,"flow_rate":null,"s_min":0.184,"s_max":3.181,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC65_fit1_model1_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"5.0","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC65_fit1_model1.pdb","model_mw":45.468,"bead_radius":3.0,"log":null,"symmetry":"P1","comment":"Average of 10 models generated by DAMMIN","user":161}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC65_fit1_fit_img.png","software":"DAMMIN","chi_square_value":0.681,"p_value":0.000835,"fit_residual_plot":"SASDC65_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC65_fit1.fir","fit_log":null,"software_version":"5.3","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"Small angle X-ray scattering measurements (SAXS) were performed at the SAXS2 beamline located at the Brazilian Synchrotron Light Laboratory (LNLS), Campinas, São Paulo, Brazil. The X-ray scattering data (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle; λ = 0.1488 nm) were acquired using a two-dimension position-sensitive MARR-CCD detector and a sample-to-detector distance of ~1000 mm, corresponding to the q-range 0 < q < 0.35. In order to checking for protein aggregation or concentration-dependent effects, samples were prepared at various protein concentrations (2 mg/mL and 3 mg/mL) in 25 mM Tris-HCl (pH 7.5) buffer, containing 150 mM NaCl, 2 mM EDTA and 1 mM β-mercaptoethanol. Samples were exposed to X-ray at different time frames (5 x 30 seconds, and 180 seconds) to investigate for X-ray damage. No effects of protein concentration or X-ray damage were detected. The model depicts the averaged spatial representation of the protein (DAMFILT occupancy and volume-corrected bead model).","tags":[],"intensity_unit":null,"experimental_mw":32.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.02,"pddf_i0_error":0.000479,"guinier_i0":0.019763,"guinier_i0_error":0.0011,"pddf_rg":3.97,"pddf_rg_error":0.08,"guinier_rg":3.692,"guinier_rg_error":0.3,"pddf_dmax":13.0,"pddf_dmax_error":5.0,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":4,"guinier_point_last":15,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC65_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}