{"code":"SASDC66","status":"Published","type_of_curve":"Extrapolated to infinite dilution","angular_unit":"1/nm","project":{"title":"The switch that does not flip: the blue-light receptor YtvA from Bacillus subtilis adopts an elongated dimer conformation independent of the activation state as revealed by a combined AUC and SAXS study.","publication":{"title":"The switch that does not flip: the blue-light receptor YtvA from Bacillus subtilis adopts an elongated dimer conformation independent of the activation state as revealed by a combined AUC and SAXS study.","author_list":"Jurk M, Dorn M, Kikhney A, Svergun D, Gärtner W, Schmieder P","journal":"J Mol Biol","doi":"10.1016/j.jmb.2010.08.036","pmid":"20800068","published_date":"2010 Oct 15"},"status":"released","submitted_date":"2017-07-03","released_date":"2017-07-14"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC66.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC66.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC66_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC66_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC66_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC66_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC66.sascif","experiment":{"instrument":{"detector":{"type":"Custom-built","name":"Pilatus 1M-W","resolution":0.172},"name":"DORIS III, DESY","city":"Hamburg","country":"Germany","beamline_name":"EMBL X33","beam_geometry":"0.60","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"pfyP - Blue light photoreceptor","short_name":"YtvA","sequence":"GASFQSFGIPGQLEVIKKALDHVRVGVVITDPALEDNPIVYVNQGFVQMTGYETEEILGK\r\nNCRFLQGKHTDPAEVDNIRTALQNKEPVTVQIQNYKKDGTMFWNELNIDPMEIEDKTYFV\r\nGIQNDITKQKEYEKLLEDSLTEITALSTPIVPIRNGISALPLVGNLTEERFNSIVCTLTN\r\nILSTSKDDYLIIDLSGLAQVNEQTADQIFKLSHLLKLTGTELIITGIKPELAMKMNKLDA\r\nNFSSLKTYSNVKDAVKVLPIM","organism":"Bacillus subtilis","uniprot_code":"O34627","uniprot_range_first":2,"uniprot_range_last":261,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MASFQSFGIPGQLEVIKKALDHVRVGVVITDPALEDNPIVYVNQGFVQMTGYETEEILGK\nNCRFLQGKHTDPAEVDNIRTALQNKEPVTVQIQNYKKDGTMFWNELNIDPMEIEDKTYFV\nGIQNDITKQKEYEKLLEDSLTEITALSTPIVPIRNGISALPLVGNLTEERFNSIVCTLTN\nILSTSKDDYLIIDLSGLAQVNEQTADQIFKLSHLLKLTGTELIITGIKPELAMKMNKLDA\nNFSSLKTYSNVKDAVKVLPIM","mw":29.12,"total_mw":58.24,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"PBS + 5 mM DTT","concentration_unit":null,"comment":"10 mM phosphate buffer; 2.7 mM KCl; 137 mM NaCl; 5 mM DTT","additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Blue light receptor YtvA, lit/active state","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":false},"contributor":[{"affiliation":[{"short_name":null,"address":"Berlin, Germany","full_name":"Leibniz-Institut für Molekulare Pharmakologie","webpage":"http://www.leibniz-fmp.de"}],"contributor_name":"Marcel","contributor_surname":"Jurk","orcid":null},{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Al","contributor_surname":"Kikhney","orcid":"https://orcid.org/0000-0003-1321-3956"}],"concentration_method":null,"concentration_unit":null,"date":"2009-10-24","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":30.0,"number_of_frames":4,"wavelength":0.15,"sample_detector_distance":2.7,"concentration_min":1.7,"concentration_max":11.0,"sample_volume":null,"flow_rate":null,"s_min":0.077,"s_max":6.237,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC66_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC66_fit1_model1.pdb","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":"P2","comment":"","user":225}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC66_fit1_fit_img.png","software":"GASBOR","chi_square_value":9.07,"p_value":0.0,"fit_residual_plot":"SASDC66_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC66_fit1.fir","fit_log":null,"software_version":"","description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC66_fit2_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC66_fit2_model1.pdb","model_mw":47.1,"bead_radius":2.2,"log":null,"symmetry":"P2","comment":"","user":225},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC66_fit2_model2_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC66_fit2_model2.pdb","model_mw":46.83,"bead_radius":3.25,"log":null,"symmetry":"","comment":"","user":225}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC66_fit2_fit_img.png","software":"DAMMIF","chi_square_value":7.896,"p_value":0.0,"fit_residual_plot":"SASDC66_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC66_fit2.fir","fit_log":null,"software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":null,"tags":["X33"],"intensity_unit":null,"experimental_mw":46.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":46.0,"porod_mw_error":null,"pddf_i0":68.65,"pddf_i0_error":null,"guinier_i0":69.76,"guinier_i0_error":null,"pddf_rg":3.178,"pddf_rg_error":null,"guinier_rg":3.18,"guinier_rg_error":0.1,"pddf_dmax":10.1,"pddf_dmax_error":1.0,"porod_volume":74.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":28,"guinier_point_last":113,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC66_datloglog_img.png","symmetry":null,"last_modified":"2023-05-25T11:21:52.654103+02:00","bragg_peak":[]}