{"code":"SASDC75","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Structural and functional studies of the Leishmania braziliensis SGT co-chaperone indicate that it shares structural features with HIP and can interact with both Hsp90 and Hsp70 with similar affinities.","publication":{"title":"Structural and functional studies of the Leishmania braziliensis SGT co-chaperone indicate that it shares structural features with HIP and can interact with both Hsp90 and Hsp70 with similar affinities.","author_list":"Coto ALS, Seraphim TV, Batista FAH, Dores-Silva PR, Barranco ABF, Teixeira FR, Gava LM, Borges JC","journal":"Int J Biol Macromol","doi":"10.1016/j.ijbiomac.2018.06.123","pmid":"29959008","published_date":"2018 Oct 15"},"status":"released","submitted_date":"2017-06-17","released_date":"2018-07-03"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC75.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC75.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC75_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC75_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC75_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC75_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC75.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"MAR 165 CCD","resolution":null},"name":"Brazilian Synchrotron Light Laboratory","city":"Campinas","country":"Brazil","beamline_name":"SAXS2 Beamline","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"SGT protein","short_name":"LbSGT","sequence":"GSHMEEKDQPITDDHRKLVFSFLRMMRRSKVENPERTEAVAQMLGEEFGVDPAGSGGLHDTEV\r\nDVLEAFKTALREHERRSGAEQDEKFMSFVELLEKKGYFKGVEKGSDEYAQRLEKARDKFN\r\nQRNNPYEGLTAEQIKNKGNELMSQAKYKEAIAYYTKAIELESDNAVFFANRAAAHTHLKD\r\nYSNAIIDCERAIVINPEYSKSYSRLGTALFYQENYARAVEAFTKACDLDPDNDTYKEDLK\r\nRAEEKAKMTGLTTGGGMGGFPGMGGFPGMGGMPDMSQFASMMNNPQFMETAQRMMQNPEF\r\nSNMVANMAGKFSQGGMDPAELNRLGADMGMRNVDEEGNVVTPFGKVNRAAIEKLQEEEVR\r\nKNPKLAGIMADVQANGYGAFQKYLGDPDVMSLMMKFQSLMFTNPNSSA","organism":"Leishmania braziliensis","uniprot_code":"A4HIK6","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MEEKDQPITDDHRKLVFSFLRMMRRSKVENPERTEAVAQMLGEEFGVDPAGSGGLHDTEV\nDVLEAFKTALREHERRSGAEQDEKFMSFVELLEKKGYFKGVEKGSDEYAQRLEKARDKFN\nQRNNPYEGLTAEQIKNKGNELMSQAKYKEAIAYYTKAIELESDNAVFFANRAAAHTHLKD\nYSNAIIDCERAIVINPEYSKSYSRLGTALFYQENYARAVEAFTKACDLDPDNDTYKEDLK\nRAEEKAKMTGLTTGGGMGGFPGMGGFPGMGGMPDMSQFASMMNNPQFMETAQRMMQNPEF\nSNMVANMAGKFSQGGMDPAELNRLGADMGMRNVDEEGNVVTPFGKVNRAAIEKLQEEEVR\nKNPKLAGIMADVQANGYGAFQKYLGDPDVMSLMMKFQSLMFTNPNSSA","mw":45.935,"total_mw":91.87,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Potassium Phosphate, 100 mM KCl, 10 mM EDTA, 1 mM B-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Leishmania braziliensis SGT co-chaperone","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Júlio","contributor_surname":"Borges","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2014-05-14","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":300.0,"number_of_frames":3,"wavelength":0.1488,"sample_detector_distance":1.0,"concentration_min":1.5,"concentration_max":6.0,"sample_volume":null,"flow_rate":null,"s_min":0.154,"s_max":3.329,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC75_fit1_model1_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"4.1","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC75_fit1_model1.pdb","model_mw":71.6,"bead_radius":null,"log":null,"symmetry":"P2","comment":"","user":161}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC75_fit1_fit_img.png","software":"DAMMIN","chi_square_value":1.476,"p_value":0.203609,"fit_residual_plot":"SASDC75_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC75_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/SGT_1.log","software_version":"5.3","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS experiments were performed at the D02A-SAXS2 beamline at the Brazilian Synchrotron Light Laboratory (LNLS, Campinas, São Paulo, Brazil). The X-ray scattering data (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle; λ = 0.1488 nm) were acquired using a two-dimensional position-sensitive MAR-CCD detector. Measurements were performed using a monochromatic X-ray beam and a sample-to-detector distance of ~1,000 mm, corresponding to the scattering vector range of 0.015 < q < 0.35 Å-1. LbSGT samples were prepared at 1.5 mg/mL, 3.0 mg/mL, 4.5 mg/mL and 6.0 mg/mL. Successive frames (20, 60 and 300 sec) were collected to inspect for X-ray damage. Aggregation or inter-particle interference were not observed. The model depicts the averaged spatial representation of the protein (DAMFILT occupancy and volume-corrected bead model).","tags":[],"intensity_unit":null,"experimental_mw":91.0,"experimental_mw_error":null,"guinier_i0_mw":102.0,"guinier_i0_mw_error":4.0,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.06631,"pddf_i0_error":0.00296,"guinier_i0":0.0659072,"guinier_i0_error":0.00039,"pddf_rg":4.66,"pddf_rg_error":0.3,"guinier_rg":4.48,"guinier_rg_error":0.4,"pddf_dmax":17.0,"pddf_dmax_error":5.0,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":2,"guinier_point_last":11,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC75_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}